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Synthetic peptide derived from residues 50 - 150 of Human TDP1.
Our Abpromise guarantee covers the use of ab4166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21041670|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
Abcam recommends blocking with 1-3% Milk.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. 20 min in boiling Tris-EDTA solution, see Abreview by Frédéric Leduc.|
Western blot using ab4166 at 1/1000 dilution for 1 hr at room temp. The lanes are as follows, from left to right. The blot was developed using ECL.
2. 1ng purified recombinant human TDP1
3. 3ng purified recombinant human TDP1
4. 6ng purified recomninant human TDP1
5. 6 micrograms total protein from MCF7 human cell extract.