製品の概要

  • 製品名Anti-TCTP antibody
    TCTP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TCTP
  • 特異性ab65015 detects endogenous levels of total TCTP protein.
  • アプリケーション適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human TCTP.

  • ポジティブ・コントロール
    • Human breast carcinoma tissue. Extracts from HepG2, COLO205 and HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab65015 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能Involved in calcium binding and microtubule stabilization.
  • 組織特異性Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.
  • 配列類似性Belongs to the TCTP family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ27337 antibody
    • Fortilin antibody
    • Histamine releasing factor antibody
    • Histamine-releasing factor antibody
    • HRF antibody
    • Immunoglobulin E-dependent antibody
    • p02 antibody
    • p23 antibody
    • TCTP antibody
    • TCTP_HUMAN antibody
    • tpt1 antibody
    • Translationally controlled tumor protein antibody
    • Translationally-controlled tumor protein antibody
    • Tumor protein translationally controlled 1 antibody
    • tumor protein, translationally-controlled 1 antibody
    see all

Anti-TCTP antibody 画像

  • ab65015, at 1/50 - 1/100 dilution, staining TCTP in human breast carcinoma by Immunohistochemistry, using paraffin-embedded tissue, in the absence (left panel) or presence (right panel) of the immunising peptide. Antigen retrieval performed by boiling in a pressure cooker for 2-3 min. A polymer system was used for signal enhancing. Stained using DAB.
  • All lanes : Anti-TCTP antibody (ab65015) at 1/500 dilution

    Lane 1 : HepG2 cell extract (5-30 ug total protein)
    Lane 2 : COLO205 cell extract (5-30 ug total protein)
    Lane 3 : HeLa cell extract (5-30 ug total protein)
    Lane 4 : HeLa cell extract with immunising peptide (5-10 ug)


    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • ICC/IF image of ab65015 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TCTP antibody (ab65015) 使用論文

ab65015 has not yet been referenced specifically in any publications.

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We have four polyclonal anti TCTP antibodies in our catalog, three are currently tested and guaranteed for WB.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"