製品の概要

  • 製品名Anti-TCTP antibody
    TCTP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TCTP
  • アプリケーション適用あり: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Rabbit, Chicken, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TCTP.

    (Peptide available as ab37505.)

  • ポジティブ・コントロール
    • This antibody gave a positive control in the follwing Human whole cell lysates: HeLa Jurkat A431 HEK 293 HepG2 MCF-7 U2OS This antibody gave a positive control in the follwing Mouse whole cell lysates: NIH 3T3 MEF1 This antibody gave a positive control in the follwing Mouse tissue lysates: Pancreas Testis Spinal Cord Ovary This antibody gave a positive control in the follwing Rat whole cell lysate: PC12 This antibody gave a positive control in the follwing Rat tissue lysate: Liver

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/2000.

ターゲット情報

Anti-TCTP antibody 画像

  • All lanes : Anti-TCTP antibody (ab37506) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : HEK293 whole cell lysate (ab7902)
    Lane 5 : HepG2 whole cell lysate (ab7900)
    Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab37506 stained human HeLa cells. The cells were MeOH fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37506, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-TCTP antibody (ab37506) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Mouse pancreas tissue lysate - total protein (ab29363)
    Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 5 : Spinal Cord (Mouse) Tissue Lysate
    Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 8 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

Anti-TCTP antibody (ab37506) 使用論文

This product has been referenced in:
  • Katsogiannou M  et al. The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets. Mol Cell Proteomics 13:3585-601 (2014). WB . Read more (PubMed: 25277244) »
  • Zhang J  et al. Role of the translationally controlled tumor protein in DNA damage sensing and repair. Proc Natl Acad Sci U S A 109:E926-33 (2012). WB . Read more (PubMed: 22451927) »

See all 10 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Mouse Cell (Hep G2)
Specification Hep G2
Permeabilization Yes - triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 23 2014

Thank you for your inquiry.

We have four polyclonal anti TCTP antibodies in our catalog, three are currently tested and guaranteed for WB.

In order to help you with your inquiry I will need the product number (starts with ab and the...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (Madin Darby Bovine Kidney cells)
Loading amount 100000 cells
Specification Madin Darby Bovine Kidney cells
Treatment Drug treatment and virus infection
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 16 2012

Thank you for contacting usyesterday and sorry for the delay in getting back to you.

As discussed over the phone, the anti-NTCTP antibody (ab37506) has not as yet been used in detecting thebovine protein as you are hoping to do. This does not...

Read More

Thank you for your enquiry. We don't know of any published papers where this antibody has been used in IHC. However we do have 2 Abrevies, customer submitted reviews, linked to the datasheet for ab37506 in which the researchers used the antibody for IH...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Genitourinary section)
Specification Genitourinary section
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Apr 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"