製品の概要

  • 製品名
    Anti-TCR V beta 5 antibody [MEM-262]
  • 製品の詳細
    Mouse monoclonal [MEM-262] to TCR V beta 5
  • 特異性
    The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
  • アプリケーション
    適用あり: Flow Cyt, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human thymoma cell line HPB-ALL.

  • 特記事項
    Functional Application Activates T cells (V beta 5-related subset).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    >95% by SDS-PAGE
  • 特記事項(精製)
    Purified from hybridoma culture supernatant by protein A affinity chromatography.
  • ポリ/モノ
    モノクローナル
  • クローン名
    MEM-262
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IP
WB
  • 追加情報
    Flow Cyt: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.
    • 細胞内局在
      Cell Membrane
    • 参照データベース
    • 別名
      • T cell receptor antibody
      • TCB antibody
      • TCRB antibody
      • TRB antibody
      • TRB@ antibody
      see all

    参考文献

    This product has been referenced in:
    • Wang CT  et al. Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats. J Formos Med Assoc 114:328-38 (2015). Read more (PubMed: 25839766) »
    • Grabherr MG  et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). Read more (PubMed: 21625601) »

    See all 3 Publications for this product

    レビューと Q&A

    Thank you for your patience. For ab9219, the originator has recommended the following. Since the antibody recognizes human cytokeratin 18 we recommend using the PBS-insoluble fraction (pellet) of human cell lines containing cytokeratin 18 (for example...

    Read More

    Thank you for your enquiry. The originator of ab1898 was able to provide me with the following information. Western blot detection: -material: HPB-ALL cell line -non-reducing conditions -detects only free beta chains -molecular weight 36 kDa ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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