製品の概要

  • 製品名Anti-TCR V beta 10 antibody [G101]
  • 製品の詳細
    Mouse monoclonal [G101] to TCR V beta 10
  • 特異性Approximately 4.2% of unseparated LEW lymph node cells were detected by this clone. Reactivity is restricted to alpha/beta T cells, of which 6.7% labelled.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Tissue/ cell preparation (Rat): T cell clone expressing the TCR V beta 10 gene product

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)ab23348 has been purified by ion exchange chromatography from tissue culture supernatant.
  • ポリ/モノモノクローナル
  • クローン名G101
  • ミエローマP3x63-Ag8.653
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab23348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報Flow Cyt: Use 10µl for 106 cells in 100µl, or 1/50 - 1/100.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas.
    • 細胞内局在Plasma membrane
    • 参照データベース

    Anti-TCR V beta 10 antibody [G101] (ab23348) 使用論文

    This product has been referenced in:
    • Torres-Nagel NE  et al. Identification of rat Tcrb-V8.2, 8.5, and 10 gene products by monoclonal antibodies. Immunogenetics 37:305-8 (1993). Read more (PubMed: 8420840) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab23348.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"