製品の概要

  • 製品名Anti-TCR alpha + TCR beta antibody [TCR2] (FITC)
    TCR alpha + TCR beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TCR2] to TCR alpha + TCR beta (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性ab25678 recognises Chicken TCR aß (Vß1).
  • アプリケーション適用あり: Functional Studies, Flow Cyt, IHC-Fr, IPmore details
  • 種交差性
    交差種: Chicken
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項


    ab25678 reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74±2%) or CD8 (26±4%). TCR2+ cells in the spleen also express either CD4 (37±1%) or CD8 (64±4%). Surprisingly, a relatively large subpopulation of CD8 + cells in the spleen are negative for TCR2. This observation led to the demonstration that 71±6% of the TCR1+ cells in the spleen express the CD8 marker.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • 一次抗体 備考ab25678 reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74±2%) or CD8 (26±4%). TCR2+ cells in the spleen also express either CD4 (37±1%) or CD8 (64±4%). Surprisingly, a relatively large subpopulation of CD8 + cells in the spleen are negative for TCR2. This observation led to the demonstration that 71±6% of the TCR1+ cells in the spleen express the CD8 marker.
  • ポリ/モノモノクローナル
  • クローン名TCR2
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

In ovo suppression of the development of TCR-Vbeta1 cells.

Flow Cyt Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • 細胞内局在Type I membrane protein.
  • 別名
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

Anti-TCR alpha + TCR beta antibody [TCR2] (FITC) 画像

  • Chicken PBMC were double stained with ab25678 (1µg/106 cells) and mouse anti-chicken CD3-R-PE. Small lymphocytes were gated and subjected to 2-color analysis on a FACScan.

Anti-TCR alpha + TCR beta antibody [TCR2] (FITC) (ab25678) 使用論文

This product has been referenced in:

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"