Anti-TCR alpha 抗体 (ab18861)
Key features and details
- Rabbit polyclonal to TCR alpha
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TCR alpha antibody
TCR alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TCR alpha -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Human)
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 5648 TCR alpha Antibody. 5648-100 is the same size as the 100 µg size of ab18861.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18861の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 16 kDa).
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特記事項 |
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 16 kDa). |
ターゲット情報
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関連性
TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR. -
細胞内局在
Plasma membrane -
参照データベース
- Entrez Gene: 6955 Human
- Omim: 186880 Human
- SwissProt: P01848 Human
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別名
- T cell antigen receptor alpha polypeptide antibody
- T cell receptor alpha antibody
- T cell receptor alpha locus antibody
see all
画像
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All lanes : Anti-TCR alpha antibody (ab18861) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
TCR alpha contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab18861 は 4 報の論文で使用されています。
- Hu Y et al. Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer. Front Oncol 12:845765 (2022). PubMed: 35936748
- Jiang Y et al. Single-cell RNA sequencing reveals TCR+ macrophages in HPV-related head and neck squamous cell carcinoma. Front Immunol 13:1030222 (2022). PubMed: 36389736
- Liu Y et al. ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation. Cell Mol Immunol 18:1904-1919 (2021). PubMed: 32572163
- Liu Y et al. Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1(+)Treg cells. Nat Commun 8:14709 (2017). IF ; Mouse . PubMed: 28436428