製品の概要

  • 製品名Anti-TCP1 delta antibody
    TCP1 delta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TCP1 delta
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide: AGLNPISTVT ELRNRHAQGE KTAGINVRKG GISNILEELV VQPLLVSVSA, corresponding to a region within C terminal amino acids 469-518 of Human TCP1 delta

  • ポジティブ・コントロール
    • HepG2 cell lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
ICC/IF 1/200.
IHC-P 1/100.

ターゲット情報

  • 関連性The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.
  • 細胞内局在Cytoplasmic > Cytoskeleton
  • 参照データベース
  • 別名
    • CCT delta antibody
    • CCT4 antibody
    • CCTD antibody
    • chaperonin containing t-complex polypeptide 1, delta subunit antibody
    • chaperonin containing TCP1, subunit 4 (delta) antibody
    • SRB antibody
    • Stimulator of TAR RNA binding antibody
    • T complex protein 1 subunit delta antibody
    • TCP-1-delta antibody
    see all

Anti-TCP1 delta antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling TCP1 delta with ab49151 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TCP1. Right - Merge.


  • Predicted band size : 60 kDa
    Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1µg/ml.
  • Immunocytochemistry/immunofluorescence analysis of Rat hippocampal neurons - 14DIV labeling TCP1 delta with ab49151 at 1/200. A Cy3-conjugated anti rabbit IgG (1/500) was used as the secondary antibody.


  • Predicted band size : 60 kDa
    Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1:1000. A Cy3-conjugated anti-rabbit IgG (1:10000) was used as the secondary antibody.
  • All lanes : Anti-TCP1 delta antibody (ab49151) at 1.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : molecular marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-TCP1 delta antibody (ab49151) 使用論文

This product has been referenced in:
  • Mallawaaratchy DM  et al. The phosphoinositide 3-kinase inhibitor LY294002, decreases aminoacyl-tRNA synthetases, chaperones and glycolytic enzymes in human HT-29 colorectal cancer cells. J Proteomics 75:1590-9 (2012). WB ; Human . Read more (PubMed: 22172953) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"