リコンビナント
RabMAb

Anti-TCP1 beta 抗体 [EPR4084] (ab92746)

製品の概要

  • 製品名
    Anti-TCP1 beta antibody [EPR4084]
    TCP1 beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4084] to TCP1 beta
  • アプリケーション
    適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C terminus of Human TCP1 beta.

  • ポジティブ・コントロール
    • WB: HeLa, MCF-7 and Daudi cell lysates IHC-P: Human colon tissue, Human kidney tissue ICC/IF: HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-TCP1 beta antibody (HRP) [EPR4084] (ab203463)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • バッファー
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR4084
  • アイソタイプ
    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab92746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能
    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • 配列類似性
    Belongs to the TCP-1 chaperonin family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 99D8.1 antibody
    • CCT 2 antibody
    • CCT beta antibody
    • CCT-beta antibody
    • CCT2 antibody
    • CCTB antibody
    • Chaperonin containing t complex polypeptide 1 beta subunit antibody
    • Chaperonin containing t complex polypeptide 1 subunit 2 antibody
    • Chaperonin containing TCP1 subunit 2 antibody
    • Chaperonin containing TCP1 subunit 2 (beta) antibody
    • CTP:phosphocholine cytidylyltransferase 2 antibody
    • Epididymis secretory sperm binding protein Li 100n antibody
    • HEL S 100n antibody
    • MGC142074 antibody
    • MGC142076 antibody
    • MGC94480 antibody
    • PRO1633 antibody
    • T complex protein 1 beta subunit antibody
    • T complex protein 1 subunit beta antibody
    • T-complex protein 1 subunit beta antibody
    • TCP 1 beta antibody
    • TCP-1-beta antibody
    • TCPB_HUMAN antibody
    see all

Anti-TCP1 beta antibody [EPR4084] 画像

  • All lanes : Anti-TCP1 beta antibody [EPR4084] (ab92746) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : Daudi cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 57 kDa
  • ab92746, at a 1/100 dilution, staining TCP1 beta in formalin fixed, paraffin embedded (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry. Detection: DAB staining.
  • ab92746, at a 1/100 dilution, staining TCP1 beta in HeLa cells by Immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab92746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92746, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-TCP1 beta antibody [EPR4084] (ab92746) 使用論文

This product has been referenced in:
  • Noormohammadi A  et al. Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan. Nat Commun 7:13649 (2016). WB ; Human . Read more (PubMed: 27892468) »
  • Shen W  et al. 2'-Fluoro-modified phosphorothioate oligonucleotide can cause rapid degradation of P54nrb and PSF. Nucleic Acids Res 43:4569-78 (2015). Read more (PubMed: 25855809) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab92746.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録