Synthetic peptide corresponding to residues in Human TCP1 alpha.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab109126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | 1/50 - 1/100. | |
WB | 1/1000 - 1/10000. Predicted molecular weight: 60 kDa. | |
IP | 1/10 - 1/100. | |
IHC-P | 1/100 - 1/250. |
ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate.
Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate
For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"