Anti-TCP1 alpha 抗体 [EPR4082] (ab109126)
製品の概要
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製品名Anti-TCP1 alpha antibody [EPR4082]
TCP1 alpha 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR4082] to TCP1 alpha
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WB, IP, IHC-Pmore details
適用なし: Flow Cyt -
種交差性交差種: Mouse, Rat, Rabbit, Human
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免疫原
Synthetic peptide within Human TCP1 alpha aa 500-600. The exact sequence is proprietary.
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ポジティブ･コントロール
- HeLa, ECV-304, MCF7 and Daudi cell lysates; Human testis tissue
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR4082
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アイソタイプIgG
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab109126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/50 - 1/100. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 60 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. |
ターゲット情報
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機能Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
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配列類似性Belongs to the TCP-1 chaperonin family.
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細胞内局在Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
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参照データベース
- Entrez Gene: 6950 Human
- Entrez Gene: 21454 Mouse
- Entrez Gene: 100302435 Rabbit
- Entrez Gene: 24818 Rat
- Omim: 186980 Human
- SwissProt: P17987 Human
- SwissProt: P11983 Mouse
- SwissProt: P28480 Rat
see all -
別名
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
see all
画像
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![Immunoprecipitation - Anti-TCP1 alpha antibody [EPR4082] (ab109126)](http://a.abcam.com/ps/products/109/ab109126/Images/ab109126-294607-ab109126IPECV304.jpg)
Immunoprecipitation - Anti-TCP1 alpha antibody [EPR4082] (ab109126)ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate.
Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate
For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-TCP1 alpha antibody [EPR4082] (ab109126) at 1/1000 dilution
Lane 1 : ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2 : PBS instead of ab109126 in ECV-304 whole cell lysate
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 60 kDa (why is the actual band size different from the predicted?) -
![Western blot - Anti-TCP1 alpha antibody [EPR4082] (ab109126)](http://a.abcam.com/ps/products/109/ab109126/Images/TCP1-alpha-Primary-antibodies-ab109126-1.jpg)
Western blot - Anti-TCP1 alpha antibody [EPR4082] (ab109126)All lanes : Anti-TCP1 alpha antibody [EPR4082] (ab109126) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : ECV-304 cell lysates
Lane 3 : MCF7 cell lysates
Lane 4 : Daudi cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha antibody [EPR4082] (ab109126)](http://a.abcam.com/ps/products/109/ab109126/Images/TCP1-alpha-Primary-antibodies-ab109126-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha antibody [EPR4082] (ab109126)ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.
プロトコール
参考文献
This product has been referenced in:
- Intawicha P et al. Proteomic profiling of rabbit embryonic stem cells derived from parthenotes and fertilized embryos. PLoS One 8:e67772 (2013). WB, ICC/IF ; Rabbit . Read more (PubMed: 23861804) »
- Gao X et al. Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins. J Biol Chem 288:25760-8 (2013). Read more (PubMed: 23888055) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"