Rabbit monoclonal [EPR4082] to TCP1 alpha
Mouse, Rat, Rabbit, Human
Synthetic peptide within Human TCP1 alpha aa 500-600. The exact sequence is proprietary.
HeLa, ECV-304, MCF7 and Daudi cell lysates; Human testis tissue
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
1/10 - 1/100.
1/100 - 1/250.
Is unsuitable for Flow Cyt.
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Belongs to the TCP-1 chaperonin family.
Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
Information by UniProt
CCT alpha antibody
Immunoprecipitation - Anti-TCP1 alpha antibody [EPR4082] (ab109126)
ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate.
Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution. Blocking and diluting buffer: 5% NFDM/TBST. All lanes :
Anti-TCP1 alpha antibody [EPR4082] (ab109126) at 1/1000 dilution
Lane 1 :
ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2 :
PBS instead of ab109126 in ECV-304 whole cell lysate
Secondary All lanes :
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size:
60 kDa (
why is the actual band size different from the predicted?
Western blot - Anti-TCP1 alpha antibody [EPR4082] (ab109126)
All lanes : Anti-TCP1 alpha antibody [EPR4082] (ab109126) at 1/1000 dilution Lane 1 : HeLa cell lysates Lane 2 : ECV-304 cell lysates Lane 3 : MCF7 cell lysates Lane 4 : Daudi cell lysates Lysates/proteins at 10 µg per lane. Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha antibody [EPR4082] (ab109126)
ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"