Anti-TCP1 alpha/CCTA 抗体 [EPR4082] (ab109126)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TCP1 alpha/CCTA antibody [EPR4082]
TCP1 alpha/CCTA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, ECV-304, MCF7 and Daudi cell lysates; Human testis tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4082 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109126の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. -
配列類似性
Belongs to the TCP-1 chaperonin family. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 6950 Human
- Entrez Gene: 21454 Mouse
- Entrez Gene: 100302435 Rabbit
- Entrez Gene: 24818 Rat
- Omim: 186980 Human
- SwissProt: P17987 Human
- SwissProt: P11983 Mouse
- SwissProt: P28480 Rat
see all -
別名
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
see all
画像
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All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : ECV-304 cell lysates
Lane 3 : MCF7 cell lysates
Lane 4 : Daudi cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa -
ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha/CCTA in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate.
Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate
For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126) at 1/1000 dilution
Lane 1 : ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2 : PBS instead of ab109126 in ECV-304 whole cell lysate
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab109126 は 4 報の論文で使用されています。
- Llamas E et al. The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress. Aging Cell 20:e13446 (2021). PubMed: 34327811
- Zhong XL et al. Low expression of TCP1 (T-Complex 1) and PSMC1 (Proteasome 26S subunit, ATPase 1) in heterotopic ossification during ankylosing spondylitis. Bioengineered 12:7459-7469 (2021). PubMed: 34612770
- Intawicha P et al. Proteomic profiling of rabbit embryonic stem cells derived from parthenotes and fertilized embryos. PLoS One 8:e67772 (2013). WB, ICC/IF ; Rabbit . PubMed: 23861804
- Gao X et al. Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins. J Biol Chem 288:25760-8 (2013). PubMed: 23888055