The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa according to NP_110379).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
ab10180 staining (1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane).Primary incubated for 1 hour.Detected by Western blot using chemiluminescence. ab10180 staining (1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
Ryan T et al. Identification of Novel Ryanodine Receptor 1 (RyR1) Protein Interaction with Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP). J Biol Chem286:17060-8 (2011).
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