保存方法 Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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精製度 Immunogen affinity purified
特記事項（精製） Affinity-purified from rabbit antiserum by affinity-chromatography using
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
機能 Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.
組織特異性 Isoform 3 is testis specific. Isoform 2 is pancreas specific.
配列類似性 Contains 1 bHLH (basic helix-loop-helix) domain.
発生段階 Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.
Information by UniProt
Cha transcription factor antibody
Anti-TCFL5 antibody 画像
Western blot - TCFL5 antibody (ab69931)
All lanes : Anti-TCFL5 antibody (ab69931) at 1/500 dilution Lane 1 : RAW264.7 cell extract Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 53 kDa Observed band size : 50 kDa ( why is the actual band size different from the predicted?) Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Anti-TCFL5 antibody (ab69931) 使用論文
has not yet been referenced specifically in any publications.
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