製品の概要

  • 製品名Anti-TCFL5 antibody
    TCFL5 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to TCFL5
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    C-KYIQERHGDSLKKE

    , corresponding to internal sequence amino acids 400-413 of Human TCFL5

  • ポジティブ・コントロール
    • Human Testis lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.
    • 組織特異性Isoform 3 is testis specific. Isoform 2 is pancreas specific.
    • 配列類似性Contains 1 bHLH (basic helix-loop-helix) domain.
    • 発生段階Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bHLHe82 antibody
      • CHA antibody
      • Cha transcription factor antibody
      • E2BP-1 antibody
      • E2BP1 antibody
      • Figlb antibody
      • HPV-16 E2-binding protein 1 antibody
      • MGC46135 antibody
      • TCFL 5 antibody
      • TCFL5 antibody
      • TCFL5_HUMAN antibody
      • transcription factor-like 5 (basic helix-loop-helix) antibody
      • Transcription factor-like 5 protein antibody
      see all

    Anti-TCFL5 antibody 画像

    • All lanes : ab53546 antibody (0.3µg/ml) staining of TCFL5 in Human Testis lysate (35µg protein in RIPA buffer).

      Lane 1 : Without the immunising peptide
      Lane 2 : With the immunising peptide


      Predicted band size : 48 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Anti-TCFL5 antibody (ab53546) 使用論文

    ab53546 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"