製品の概要

  • 製品名Anti-TCF7 antibody
    TCF7 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to TCF7
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: VIAECTLKES AAINQILGRR WHALSREEQA KYYELARKER QLHMQLYPGW SARDNYGKKK RRSREKHQES TTGGKRNAFG TYPEKAAAPA PFLPMTVL, corresponding to amino acids 287-385 of Human TCF7

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
    • 組織特異性Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
    • 配列類似性Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ36364 antibody
      • MGC47735 antibody
      • OTTHUMP00000159391 antibody
      • T cell factor 1 antibody
      • T cell specific transcription factor 1 antibody
      • T-cell factor 1 antibody
      • T-cell-specific transcription factor 1 antibody
      • TCF-1 antibody
      • TCF-7 antibody
      • TCF1 antibody
      • Tcf7 antibody
      • TCF7 transcription factor 7 antibody
      • TCF7_HUMAN antibody
      • Transcription factor 7 (T cell specific HMG box) antibody
      • Transcription factor 7 antibody
      • Transcription factor-7, T-cell specific antibody
      • Transcription factor-7, T-cell specific, 1 antibody
      see all

    Anti-TCF7 antibody 画像

    • TCF7 antibody (ab58271) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.
    • Western blot against tagged recombinant protein immunogen using ab58271 TCF7 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-TCF7 antibody (ab58271) 使用論文

    ab58271 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample Zebrafish Tissue sections (Skeleton)
    Specification Skeleton
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Mar 12 2014

    Thank you for contacting Abcam.


    I have looked at both ab58271 and ab90136 and although they have not been tested on mouse tissue, based on sequence homology there is a high chance that both of these antibodies will react to mouse tissue...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"