製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TCF4
  • 特異性
    Detects endogenous levels of total TCF4 protein.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Human TCF4 aa 581-630 (internal sequence).
    Database link: P15884
    (Peptide available as ab101669)

  • ポジティブ・コントロール
    • HUVEC extract

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ELISA 1/20000.

ターゲット情報

  • 機能
    Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
  • 組織特異性
    Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
  • 関連疾患
    Defects in TCF4 are a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954]. PTHS is a rare syndromic encephalopathy characterized by severe psychomotor delay, epilepsy, daily bouts of diurnal hyperventilation starting in infancy, mild postnatal growth retardation, postnatal microcephaly, and distinctive facial features. Since most hitherto reported cases have been sporadic, with males and females equally affected, PTHS is regarded as an autosomal dominant condition.
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • ドメイン
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHb19 antibody
    • Class B basic helix-loop-helix protein 19 antibody
    • E2 2 antibody
    • FECD3 antibody
    • Immunoglobulin transcription factor 2 antibody
    • ITF 2 antibody
    • ITF-2 antibody
    • ITF2 antibody
    • ITF2_HUMAN antibody
    • MGC149723 antibody
    • MGC149724 antibody
    • PTHS antibody
    • SEF 2 antibody
    • SEF-2 antibody
    • SEF2 1 antibody
    • SEF2 1A antibody
    • SEF2 1B antibody
    • SEF2 1D antibody
    • SEF2 antibody
    • SL3 3 enhancer factor 2 antibody
    • SL3-3 enhancer factor 2 antibody
    • TCF 4 antibody
    • TCF-4 antibody
    • TCF4 antibody
    • Transcription factor 4 antibody
    • Transcription factor 4, isoform C antibody
    • Transcription factor 4, isoform D antibody
    • Transcription factor 4, isoform E antibody
    • Transcription factor 4, isoform L antibody
    • Transcription factor 4, isoform M antibody
    • Transcription factor 4, isoform R antibody
    see all

Anti-TCF4 antibody 画像

  • All lanes : Anti-TCF4 antibody (ab72586) at 1/500 dilution

    Lane 1 : HUVEC extract
    Lane 2 : HUVEC extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 71 kDa
    Observed band size : 71 kDa

プロトコール

Anti-TCF4 antibody (ab72586) 使用論文

This product has been referenced in:
  • Kim JY  et al. CWP232228 targets liver cancer stem cells through Wnt/ß-catenin signaling: a novel therapeutic approach for liver cancer treatment. Oncotarget 7:20395-409 (2016). WB . Read more (PubMed: 26967248) »
  • Lemieux ME  et al. Inactivation of a Single Copy of Crebbp Selectively Alters Pre-mRNA Processing in Mouse Hematopoietic Stem Cells. PLoS One 6:e24153 (2011). WB ; Mouse . Read more (PubMed: 21901164) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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