Anti-TCF3 / E2A (phospho T355) 抗体 (ab85772)

製品の概要

  • 製品名Anti-TCF3 / E2A (phospho T355) antibody
    TCF3 / E2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TCF3 / E2A (phospho T355)
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Hamster, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human TCF3/ E2A, phosphorylated at T355.

    (Peptide available as ab103576.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both HeLa and HepG2 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).

ターゲット情報

  • 機能Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
  • 関連疾患Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • ドメインthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻訳後修飾Phosphorylated following NGF stimulation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGM8 antibody
    • bHLHb21 antibody
    • Class B basic helix-loop-helix protein 21 antibody
    • E12 antibody
    • E2A antibody
    • E2A immunoglobulin enhancer binding factors E12/E47 antibody
    • E47 antibody
    • Helix loop helix protein HE47 antibody
    • Immunoglobulin enhancer-binding factor E12/E47 antibody
    • Immunoglobulin transcription factor 1 antibody
    • ITF1 antibody
    • Kappa-E2-binding factor antibody
    • MGC129647 antibody
    • MGC129648 antibody
    • Negative vitamin D response element binding protein antibody
    • NOL1-TCF3 fusion antibody
    • TCF-3 antibody
    • Tcf3 antibody
    • TFE2_HUMAN antibody
    • transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) antibody
    • Transcription factor 3 antibody
    • transcription factor 3 variant 3 antibody
    • Transcription factor E2-alpha antibody
    • Transcription factor ITF-1 antibody
    • VDIR antibody
    • VDR interacting repressor antibody
    • vitamin D receptor-interacting repressor antibody
    see all

Anti-TCF3 / E2A (phospho T355) antibody 画像

  • All lanes : Anti-TCF3 / E2A (phospho T355) antibody (ab85772) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-TCF3 / E2A (phospho T355) antibody (ab85772) 使用論文

ab85772 has not yet been referenced specifically in any publications.

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