製品の概要

  • 製品名Anti-TCF3 / E2A antibody
    TCF3 / E2A 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to TCF3 / E2A
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide from an internal region:

    KAPRARTSPDEDED

    by a Cysteine residue linker, corresponding to amino acids 522-535 of Human TCF3/ E2A

  • ポジティブ・コントロール
    • Daudi cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/128000.

ターゲット情報

  • 機能Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
  • 関連疾患Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • ドメインthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻訳後修飾Phosphorylated following NGF stimulation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGM8 antibody
    • bHLHb21 antibody
    • Class B basic helix-loop-helix protein 21 antibody
    • E12 antibody
    • E2A antibody
    • E2A immunoglobulin enhancer binding factors E12/E47 antibody
    • E47 antibody
    • Helix loop helix protein HE47 antibody
    • Immunoglobulin enhancer-binding factor E12/E47 antibody
    • Immunoglobulin transcription factor 1 antibody
    • ITF1 antibody
    • Kappa-E2-binding factor antibody
    • MGC129647 antibody
    • MGC129648 antibody
    • Negative vitamin D response element binding protein antibody
    • NOL1-TCF3 fusion antibody
    • TCF-3 antibody
    • Tcf3 antibody
    • TFE2_HUMAN antibody
    • transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) antibody
    • Transcription factor 3 antibody
    • transcription factor 3 variant 3 antibody
    • Transcription factor E2-alpha antibody
    • Transcription factor ITF-1 antibody
    • VDIR antibody
    • VDR interacting repressor antibody
    • vitamin D receptor-interacting repressor antibody
    see all

Anti-TCF3 / E2A antibody 画像

  • Anti-TCF3 / E2A antibody (ab59117) at 0.01 µg/ml + Daudi cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab59117 (3.8 µg/ml) staining TCF3/E2A in paraffin-embedded human kidney tissue following steamed antigen retrival with citrate buffer (pH 6).

Anti-TCF3 / E2A antibody (ab59117) 使用論文

ab59117 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic Stem Cells)
Permeabilization Yes - 0.2% TritonX
Specification Embryonic Stem Cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde
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投稿 Aug 10 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"