製品の概要
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製品名Anti-TCF3 / E2A antibody
TCF3 / E2A 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to TCF3 / E2A
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由来種Goat
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アプリケーション適用あり: WB, IHC-P, ELISAmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide from an internal region:
KAPRARTSPDEDED
by a Cysteine residue linker, corresponding to amino acids 522-535 of Human TCF3/ E2A -
ポジティブ･コントロール
- Daudi cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab59117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa). | |
| IHC-P | Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ELISA | 1/128000. |
ターゲット情報
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機能Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
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関連疾患Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
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配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
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ドメインthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
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翻訳後修飾Phosphorylated following NGF stimulation.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 6929 Human
- Entrez Gene: 21423 Mouse
- Entrez Gene: 171046 Rat
- Omim: 147141 Human
- SwissProt: P15923 Human
- SwissProt: P15806 Mouse
- SwissProt: P21677 Rat
- Unigene: 371282 Human
see all -
別名
- AGM8 antibody
- bHLHb21 antibody
- Class B basic helix-loop-helix protein 21 antibody
see all
画像
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Western blot - Anti-TCF3 / E2A antibody (ab59117)Anti-TCF3 / E2A antibody (ab59117) at 0.01 µg/ml + Daudi cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody (ab59117)ab59117 (3.8 µg/ml) staining TCF3/E2A in paraffin-embedded human kidney tissue following steamed antigen retrival with citrate buffer (pH 6).
参考文献
ab59117 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"