製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TCF15
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide within Human TCF15 aa 1-50 (N terminal). The exact sequence is proprietary.
    Sequence:

    AFALLRPVGAHVLYPDVRLLSEDEENRSESDASDQSFGCCEGPEAARRGP


    Database link: Q12870

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

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  • Anti-TCF15 antibody (ab66371) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab66371 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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