製品の概要

  • 製品名Anti-TC10 antibody [Y304]
    TC10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y304] to TC10
  • 特異性ab32079 recognises TC10.
  • アプリケーション適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to the third GTP unit of human TC10

  • ポジティブ・コントロール
    • 293 cell lysate, HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
ICC/IF 1/250 - 1/500.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.
    • 配列類似性Belongs to the small GTPase superfamily. Rho family.
    • 翻訳後修飾May be post-translationally modified by both palmitoylation and polyisoprenylation.
    • 細胞内局在Cytoplasm. Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ARHQ antibody
      • Ras homolog gene family member Q antibody
      • RAS like family 7 member A antibody
      • Ras like protein family member 7A antibody
      • Ras like protein TC10 antibody
      • Ras related GTP binding protein TC10 antibody
      • Ras-like protein family member 7A antibody
      • Ras-like protein TC10 antibody
      • RASL7A antibody
      • Rho related GTP binding protein RhoQ antibody
      • Rho-related GTP-binding protein RhoQ antibody
      • Rhoq antibody
      • RHOQ_HUMAN antibody
      • TC10A antibody
      see all

    Anti-TC10 antibody [Y304] 画像

    • Anti-TC10 antibody [Y304] (ab32079) at 1/1000 dilution + 293 cell lysate

      Predicted band size : 23 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent analysis of TC10 expression in HeLa cells using 1/250 ab 32079.

    Anti-TC10 antibody [Y304] (ab32079) 使用論文

    This product has been referenced in:
    • Fujita A  et al. GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. PLoS One 8:e79689 (2013). ICC/IF . Read more (PubMed: 24223996) »
    • Heasman SJ  et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). WB ; Human . Read more (PubMed: 20733052) »

    See all 4 Publications for this product

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - other (embryonic dorsal root ganglia)
    Loading amount 30 µg
    Specification embryonic dorsal root ganglia
    Gel Running Conditions Reduced Denaturing
    Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
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    投稿 Nov 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"