製品の概要

  • 製品名Anti-Tbx6 antibody
    Tbx6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tbx6
  • アプリケーション適用あり: IHC-Fr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Tbx6.

    (Peptide available as ab26243.)

  • ポジティブ・コントロール
    • E14tG2a (Mouse embryonic stem cell line) and Mouse EGL Whole Cell Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 25157815
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).

ターゲット情報

  • 機能Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.
  • 組織特異性Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.
  • 配列類似性Contains 1 T-box DNA-binding domain.
  • 発生段階Expressed during gastrulation and during a second phase in some adult tissues.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DFNB 67 antibody
    • DFNB67 antibody
    • SCDO5 antibody
    • T box 6 antibody
    • T box protein 6 antibody
    • T box transcription factor TBX 6 antibody
    • T box transcription factor TBX6 antibody
    • T-box protein 6 antibody
    • T-box transcription factor TBX6 antibody
    • Tbox 6 antibody
    • TBX 6 antibody
    • tbx6 antibody
    • TBX6_HUMAN antibody
    see all

Anti-Tbx6 antibody 画像

  • All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate
    Lane 3 : Human testis tissue lysate - total protein (ab30257)
    Lane 4 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC-P image of Tbx6 staining with ab38883 on tissue sections from an E8.5 mouse embryo. The sections were Paraformaldehyde fixed, and permeabilized in PBST (PBS/0.5% v/v TritonX100), before incubation with ab38883 (1/500 dilution) for 48 hours at 4°C in 1% BSA, 5% donkey serum in PBST. The secondary used was an Alexa-Fluor 555 conjugated donkey anti-rabbit polyclonal, at a 1/1000 dilution.

    See Abreview

Anti-Tbx6 antibody (ab38883) 使用論文

This product has been referenced in:
  • Chal J  et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016). Read more (PubMed: 27583644) »
  • Gouti M  et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol 12:e1001937 (2014). ICC/IF ; Mouse . Read more (PubMed: 25157815) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E8.5 mouse embryo section)
Specification E8.5 mouse embryo section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS/0.5% v/v TritonX100 (Sigma)
Blocking step (agent) for 30 minute(s) · Concentration: 100%
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投稿 Aug 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (tail bud e12.5)
Loading amount 5 µg
Specification tail bud e12.5
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
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投稿 Aug 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"