製品の概要

  • 製品名Anti-TBX5 antibody
    TBX5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TBX5
  • 特異性This antibody is specific for TBX5.
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide found within the following sequence: NSMHKYQPRL HIVKADENNG FGSKNTAFCT HVFPETAFIA VTSYQNHKIT (Human)

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-TBX5 antibody 画像

  • Lane 1 : Marker
    Lane 2 : Anti-TBX5 antibody (ab49308) at 5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg/ml

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 53 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of Human Brain, cortex tissue at an antibody concentration of 5.0µg/ml using anti-TBX5 antibody (ab49308)

Anti-TBX5 antibody (ab49308) 使用論文

ab49308 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 27 2014

Thank you for your question. The peptide immunogen for ab49308 has 100% homology to the Scyliorhinus canicula T-box protein 5 (Tbx5) sequence you provided. I hope this is helpful. Please contact me again if you have any further questions.

Thank you for your enquiry. Thus far I have found the following information regarding potential reactivity of our TBX5 antibodies to Scyliorhinus canicula: ab58253 - no chance for cross reactivity ab86370 - region of immunogen overlaps partially but th...

Read More

Please find below two links to our recommended protocols: One details a full nuclear extraction protocol http://www.abcam.com/ps/pdf/protocols/Nuclear%20fractionation%20protocol.pdf one details the extraction using RIPA buffer:http://www.abcam...

Read More

Thank you for your enquiry. I am sorry to hear you are having a problem with ab49308. As you mention, this antibody has so far not been tested in zebrafish but we would expect the antibody to work in this species due to the homology of the protein i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"