製品の概要
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製品名Anti-TBX5 antibody
TBX5 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TBX5
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Mouse
交差が予測される動物種: Rat, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse TBX5.
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ポジティブ･コントロール
- This antibody gave a positive signal in Mouse embryo brain (E10)tissue lysate, as well as F9 and NIH3T3 whole cell lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab101227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 24797668 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 57 kDa). |
ターゲット情報
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機能Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation.
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関連疾患Defects in TBX5 are the cause of Holt-Oram syndrome (HOS) [MIM:142900]. HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects.
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配列類似性Contains 1 T-box DNA-binding domain.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 21388 Mouse
- Entrez Gene: 304514 Rat
- SwissProt: P70326 Mouse
- SwissProt: Q5CZX7 Mouse
- SwissProt: Q5I2P1 Rat
- Unigene: 103636 Mouse
- Unigene: 6327 Rat
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別名
- Holt Oram syndrome antibody
- HOS antibody
- T box 5 antibody
see all
画像
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Western blot - TBX5 antibody (ab101227)All lanes : Anti-TBX5 antibody (ab101227) at 1 µg/ml
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : E10 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 49 kDa (why is the actual band size different from the predicted?)
Exposure time: 8 minutes
The predicted molecular weight of TBX5 is 57 kDa (SwissProt), however we expect to observe a banding pattern at 49 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
参考文献
This product has been referenced in:
- Jiang XY et al. Inhibition of Gata4 and Tbx5 by Nicotine-Mediated DNA Methylation in Myocardial Differentiation. Stem Cell Reports 8:290-304 (2017). WB, IF, Flow Cyt . Read more (PubMed: 28111280) »
- Malliaras K et al. Stimulation of endogenous cardioblasts by exogenous cell therapy after myocardial infarction. EMBO Mol Med 6:760-77 (2014). ICC/IF ; Mouse . Read more (PubMed: 24797668) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"