製品の概要

  • 製品名Anti-Tbx2 antibody
    Tbx2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tbx2
  • アプリケーション適用あり: WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Tbx2.

    (Peptide available as ab35983.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human tissue lysates: Kidney; Lung; Heart

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab33298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

Anti-Tbx2 antibody 画像

  • All lanes : Anti-Tbx2 antibody (ab33298) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • Formalin-fixed paraffin-embedded mouse bone marrow section stained with ab33298 Tbx2 antibody at 1/500 dilution.
  • ab33298 staining Tbx2 in mouse heart and somatopleure tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with methanol. Samples were then blocked with 5% serum for 2 hours at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An undiluted Cy2®-conjugated Donkey anti-rabbit IgG was used as secondary antibody.

    See Abreview

Anti-Tbx2 antibody (ab33298) 使用論文

ab33298 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart and somatopleure)
Specification Heart and somatopleure
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Jun Xu

Verified customer

投稿 May 25 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Bone Marrow)
Specification Bone Marrow
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"