製品の概要

  • 製品名Anti-TBX18 antibody
    TBX18 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TBX18
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human TBX18.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HUVEC; HepG2; MCF7; Caco2. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).

ターゲット情報

Anti-TBX18 antibody 画像

  • ab115262 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115262 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-TBX18 antibody (ab115262) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab115262 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-TBX18 antibody (ab115262) 使用論文

ab115262 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human ES cell derived epicardium-like cells)
Permeabilization Yes - saponin
Specification Human ES cell derived epicardium-like cells
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Apr 21 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"