Anti-TBX1 抗体 [EPR3289(2)] (ab109313)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3289(2)] to TBX1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TBX1 antibody [EPR3289(2)]
TBX1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3289(2)] to TBX1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Jurkat, NCCIT, PC-12, F9, C6, Neuro-2a, HEK-293, HeLa cell lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3289(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109313の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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機能
Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. -
関連疾患
Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS) [MIM:188400, 192430]. DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life.
Defects in TBX1 are a cause of DiGeorge syndrome (DGS) [MIM:188400].
Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS) [MIM:192430].
Defects in TBX1 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies. -
配列類似性
Contains 1 T-box DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6899 Human
- Entrez Gene: 21380 Mouse
- Entrez Gene: 360737 Rat
- Omim: 602054 Human
- SwissProt: O43435 Human
- SwissProt: P70323 Mouse
- Unigene: 173984 Human
- Unigene: 295194 Mouse
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別名
- Brachyury antibody
- CAFS antibody
- CTHM antibody
see all
画像
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All lanes : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/1000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) control cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) nuclear cell lysate
Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking buffer: 5% NFDM/TBST.
There is a strong non-specific band in human liver. Human liver is negative control for TBX1 which is consistent with PMID: 28920943.
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All lanes : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/2000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking buffer: 5% NFDM/TBST.
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Lane 1 : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/2000 dilution
Lane 2 : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST.
Exposure time: 30 seconds (Lane 1) and 180 seconds (Lane 2).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab109313 は 9 報の論文で使用されています。
- Lin QY et al. VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis. Clin Transl Med 11:e374 (2021). PubMed: 33783987
- Hou L et al. Dihydromyricetin Ameliorates Inflammation-Induced Insulin Resistance via Phospholipase C-CaMKK-AMPK Signal Pathway. Oxid Med Cell Longev 2021:8542809 (2021). PubMed: 34650665
- Hou L et al. Dihydromyricetin resists inflammation-induced muscle atrophy via ryanodine receptor-CaMKK-AMPK signal pathway. J Cell Mol Med 25:9953-9971 (2021). PubMed: 34676967
- Meyers K et al. Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice. Sci Rep 10:14589 (2020). PubMed: 32883997
- Cui J et al. TBX1 Functions as a Tumor Activator in Prostate Cancer by Promoting Ribosome RNA Gene Transcription. Front Oncol 10:616173 (2020). PubMed: 33575219
- Li C et al. MicroRNA-3651 promotes colorectal cancer cell proliferation through directly repressing T-box transcription factor 1. Int J Mol Med 45:956-966 (2020). PubMed: 31922246
- Velazquez-Villegas LA et al. TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue. Nat Commun 9:245 (2018). PubMed: 29339725
- Hou L et al. Browning of Pig White Preadipocytes by Co-Overexpressing Pig PGC-1a and Mice UCP1. Cell Physiol Biochem 48:556-568 (2018). PubMed: 30021212
- Yu Y et al. Smad7 enables STAT3 activation and promotes pluripotency independent of TGF-ß signaling. Proc Natl Acad Sci U S A 114:10113-10118 (2017). PubMed: 28874583