Anti-TBX1 抗体 (ab18530)
Key features and details
- Rabbit polyclonal to TBX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TBX1 antibody
TBX1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TBX1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TBX1.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18530の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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機能
Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. -
関連疾患
Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS) [MIM:188400, 192430]. DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life.
Defects in TBX1 are a cause of DiGeorge syndrome (DGS) [MIM:188400].
Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS) [MIM:192430].
Defects in TBX1 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies. -
配列類似性
Contains 1 T-box DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6899 Human
- Omim: 602054 Human
- SwissProt: O43435 Human
- Unigene: 173984 Human
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別名
- Brachyury antibody
- CAFS antibody
- CTHM antibody
see all
画像
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All lanes : Anti-TBX1 antibody (ab18530) at 1 µg/ml
Lane 1 : Human heart tissue lysate (total protein)
Lane 2 : Human skeletal muscle tissue lysate (total protein)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18530 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab18530 は 22 報の論文で使用されています。
- Condello V et al. Gene expression profile in metastatic and non-metastatic parathyroid carcinoma. Endocr Relat Cancer 28:111-134 (2021). PubMed: 33290252
- Jiang X et al. Variants in a cis-regulatory element of TBX1 in conotruncal heart defect patients impair GATA6-mediated transactivation. Orphanet J Rare Dis 16:334 (2021). PubMed: 34332615
- Caprio C et al. TBX1 and Basal Cell Carcinoma: Expression and Interactions with Gli2 and Dvl2 Signaling. Int J Mol Sci 21:N/A (2020). PubMed: 31963474
- Miller CN et al. Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages. Medicines (Basel) 6:N/A (2019). PubMed: 30709026
- Alfano D et al. Tbx1 regulates extracellular matrix-cell interactions in the second heart field. Hum Mol Genet 28:2295-2308 (2019). PubMed: 31180501