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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TBX1.
(Peptide available as ab19624.)
Our Abpromise guarantee covers the use of ab18530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21908517|
|IP||Use at an assay dependent concentration. PubMed: 25197075|
|IHC-P||Use at an assay dependent concentration. PubMed: 21177346|
ab18530 staining human U2OS cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton-100 [PBS] prior to blocking in 1% BSA for 15 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. The secondary antibody was an Alexa Fluor® 647 conjugated donkey anti-rabbit, diluted 1/1000.