製品の概要

  • 製品名Anti-TBX1 antibody
    TBX1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TBX1
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 334-364 of Human TBX1

  • ポジティブ・コントロール
    • HepG2 cell lysate, Human Testis Tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab103127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

ターゲット情報

  • 機能Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries.
  • 関連疾患Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS) [MIM:188400, 192430]. DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life.
    Defects in TBX1 are a cause of DiGeorge syndrome (DGS) [MIM:188400].
    Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS) [MIM:192430].
    Defects in TBX1 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies.
  • 配列類似性Contains 1 T-box DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Brachyury antibody
    • CAFS antibody
    • CTHM antibody
    • DGCR antibody
    • DGS antibody
    • DORV antibody
    • T box antibody
    • T box 1 antibody
    • T box 1 transcription factor antibody
    • T box 1 transcription factor C antibody
    • T box protein 1 antibody
    • T box transcription factor TBX 1 antibody
    • T box transcription factor TBX1 antibody
    • T-box protein 1 antibody
    • T-box transcription factor TBX1 antibody
    • TBX 1 antibody
    • TBX 1C antibody
    • tbx1 antibody
    • TBX1_HUMAN antibody
    • TBX1C antibody
    • Testis specific T box protein antibody
    • Testis-specific T-box protein antibody
    • TGA antibody
    • VCFS antibody
    see all

Anti-TBX1 antibody 画像

  • Immunofluorescence of A2058 cells labelling TBX1 with ab103127. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab103127 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX1 immunoreactivity is localized to the nucleus.

  • Anti-TBX1 antibody (ab103127) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 43 kDa
  • Formalin-fixed and paraffin-embedded Human testis tissue, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining, using ab103127 at a dilution of 1/50.

Anti-TBX1 antibody (ab103127) 使用論文

ab103127 has not yet been referenced specifically in any publications.

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