製品の概要

  • 製品名Anti-TBR1 antibody
    TBR1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TBR1
  • アプリケーション適用あり: ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-FoFr, IHC-P, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse TBR1.

    (Peptide available as ab25853.)

  • ポジティブ・コントロール
    • Mouse Hippocampus Tissue Lysate, Rat Hippocampus Tissue Lysate, Human cerebral cortex

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab31940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with Mouse TBR1 peptide (ab25853).
IHC-Fr 1/500.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19709629
IHC-P 1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/500.

ターゲット情報

Anti-TBR1 antibody 画像

  • IHC-P image of TBR1 staining on mouse brain sections using ab31940 (1:200). The sections were deparaffinized and subjected to heat mediated antigen retrieval. The sections were blocked using 7.5% goat serum for 2 hours at room temperature. ab31940 was diluted 1:200 using blocking buffer and incubated with the sections for 16 hours at 4°C. The secondary antibody used was Goat polyclonal to anti-rabbit conjugated to Alexa Fluor 594 (1:400). DAPI was used to counter stain nuclei.

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  • All lanes : Anti-TBR1 antibody (ab31940) at 1 µg/ml

    Lane 1 : Mouse Hippocampus Whole Cell Lysate
    Lane 2 : Rat Hippocampus Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • ab31940 staining TBR1 in human ES cells by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with Triton X-100. The sample was incubated with the primary antibody (1/200) for 30 minutes. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/20000) was used as the secondary antibody. Gating Strategy: All cells.

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  • ab31940 staining rat brain sections by IHC-Fr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected with 30% sucrose and sectioned using a cryostat. The tissue was blocked with 10% donkey serum in 0.3% TritonX in 0.1% PBS for 30 minutes at 24°C.  Staining with ab31940 at a 1/200 dilution in 0.3% TritonX with 0.1x PBS- 10% Donkey serum was performed for 4h at 24°C. A donkey anti-rabbit Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody. TBR1 expressed in the several cortical layers distinctly in pyramidal neurons.

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  • ab31940 staining mouse brain cells by ICC/IF. The cultured cells were fixed with PFA and permeabilized in PBST. The primary antibody was diluted 1/500 and incubated with the sample for 14 hours. An Alexa Fluor® 594 goat anti-rabbit antibody was used as the secondary.

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  • ab31940 staining rat cortical neurons by ICC.  The cultured cells were fixed with PFA and permeabilized in 0.1% Triton X prior to blocking in 10% BSA for 1 hour at 22°C.  The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 22°C.  A Cy2® donkey anti-rabbit antibody was used as the secondary

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  • (Image courtesy of Human Protein Atlas)
    ab31940 staining TBR1 protein in normal human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab31940(1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

Anti-TBR1 antibody (ab31940) 使用論文

This product has been referenced in:

See all 77 Publications for this product

Product Wall

Application Western blot
Sample Rabbit Tissue lysate - whole (brain)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification brain
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Praveen Ballabh

Verified customer

投稿 Nov 05 2015

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
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Abcam user community

Verified customer

投稿 Sep 09 2015

Abreviews
Application ChIP
Sample Mouse Tissue lysate - nuclear (Brain tissue, whole cortex)
Negative control During the IP step, I used a general Rabbit IgG as a negative control, which was also used to verify enrichment over input and IgG. I used a gene desert region upstream of Dlx2 as my negative control for my qPCR experiment. Also, a no template control was included to account for technical error (contamination etc).
Specification Brain tissue, whole cortex
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% PFA in 1X PBS
Positive control I used Grin2b a known direct target of Tbr1 as a positive control for my qPCR experiment to check for enrichment after my IP step.
Username

Dr. Siavash Fazel Darbandi

Verified customer

投稿 Aug 07 2015

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - tx100
Sample Human Cell (Human ES cells)
Specification Human ES cells
Gating Strategy all cells
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
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Abcam user community

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投稿 Jul 23 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample Human Cell (human iPS cell derived cortical neurons)
Specification human iPS cell derived cortical neurons
Permeabilization Yes - see blocking buffer
Fixative Paraformaldehyde
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Abcam user community

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投稿 Jul 10 2014

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jan 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E15.5 brain)
Specification E15.5 brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Abcam user community

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投稿 Nov 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Methanol
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: RT°C
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Abcam user community

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投稿 Oct 02 2012

Selon nos informations vous avez connu quelques difficultés avec de lot d'ab31940 et avez contacté notre service scientifique.

Comme nous avons pas pu expliquer le problème, nous vous avions demandé de nous four...

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Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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投稿 May 11 2012

1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"