製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TBLR1
  • 由来種
    Rabbit
  • 特異性
    Does not cross-react with TBL1.
  • アプリケーション
    適用あり: ChIP, IP, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human)

  • ポジティブ・コントロール
    • HeLa nuclear extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 56 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24446484

ターゲット情報

  • 機能
    F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the WD repeat EBI family.
    Contains 1 F-box-like domain.
    Contains 1 LisH domain.
    Contains 8 WD repeats.
  • ドメイン
    The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C21 antibody
    • DC42 antibody
    • F box like/WD repeat containing protein TBL1XR1 antibody
    • F-box-like/WD repeat-containing protein TBL1XR1 antibody
    • FLJ12894 antibody
    • IRA1 antibody
    • Nuclear receptor corepressor/HDAC3 complex subunit antibody
    • Nuclear receptor corepressor/HDAC3 complex subunit TBLR1 antibody
    • TBL1 related protein 1 antibody
    • TBL1-related protein 1 antibody
    • TBL1R_HUMAN antibody
    • TBL1XR1 antibody
    • Transducin (beta) like 1 X linked receptor 1 antibody
    • Transducin beta like 1X related protein 1 antibody
    • Transducin beta-like 1X-related protein 1 antibody
    see all

画像

  • Anti-TBLR1 antibody (ab24550) at 1/2000 dilution + HeLa nuclear extract

    Predicted band size: 56 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)

  • ab24550 used in Immunoprecipitation.
    Lysate prepared from human lung carcinoma cells was loaded at 500µg. The immunoprecipitation step was performed using Protein G. 50% of the immunoprecipitated material was loaded on each lane. Membrane was blocked with blocking buffer (PBS+ 5% non fat milk powder) for 1 hour at room temperature followed by incubation with ab24550 in blocking buffer plus 0.1% Tween-20 overnight (around 16 hours) at 4 degree centigrade. Blot was washed thrice in PBS plus 0.1% Tween-20, followed by incubation with anti-rabbit IgG HRPO in blocking buffer plus 0.1% Tween-20 at room temperature for 1 hour. Blot was washed thrice in PBS plus 0.1% Tween-20, followed by ECL detection of reactive bands.

    See Abreview

参考文献

This product has been referenced in:
  • Moretti C  et al. SLY regulates genes involved in chromatin remodeling and interacts with TBL1XR1 during sperm differentiation. Cell Death Differ 24:1029-1044 (2017). WB, IP ; Mouse . Read more (PubMed: 28475176) »
  • Daniels G  et al. Cytoplasmic, full length and novel cleaved variant, TBLR1 reduces apoptosis in prostate cancer under androgen deprivation. Oncotarget 7:39556-39571 (2016). WB . Read more (PubMed: 27127173) »

See all 7 Publications for this product

レビューと Q&A

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Application
ChIP
Detection step
Semiquantitative PCR
Sample
Mouse Tissue lysate - whole (Mouse liver)
Specification
Mouse liver
Negative control
IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37%FORMALDEHYDE
Positive control
POL ll
Username

Mr. Natarajan Balasubramaniyan

Verified customer

投稿 Sep 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (lung carcinoma)
Total protein in input
500 µg
Specification
lung carcinoma
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

投稿 Jun 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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