製品の概要
-
製品名Anti-TBL1 antibody - ChIP Grade
TBL1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TBL1 - ChIP Grade
-
由来種Rabbit
-
特異性Does not cross-react with TBLR1.
-
アプリケーション適用あり: ChIP, WB, IP, ICC/IFmore details
-
種交差性交差種: Human
-
免疫原
Recombinant full length protein (Human)
-
ポジティブ･コントロール
- HeLa nuclear extract
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
精製度Whole antiserum
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
ChIP Related Products
-
Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab24548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP | Use at an assay dependent dilution. | |
| WB | 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa). | |
| IP | Use at an assay dependent dilution. | |
| ICC/IF | 1/300. |
ターゲット情報
-
機能F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
-
組織特異性Ubiquitous.
-
配列類似性Belongs to the WD repeat EBI family.
Contains 1 F-box-like domain.
Contains 1 LisH domain.
Contains 8 WD repeats. -
ドメインThe F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
-
細胞内局在Nucleus.
- Information by UniProt
-
参照データベース
- Entrez Gene: 6907 Human
- Omim: 300196 Human
- SwissProt: O60907 Human
- Unigene: 495656 Human
-
別名
- EBI antibody
- F box like/WD repeat protein TBL1X antibody
- F-box-like/WD repeat-containing protein TBL1X antibody
see all
画像
-
Western blot - TBL1 antibody - ChIP Grade (ab24548)Anti-TBL1 antibody - ChIP Grade (ab24548) at 1/1000 dilution + HeLa nuclear extract.
Predicted band size: 63 kDa
Observed band size: 64 kDa (why is the actual band size different from the predicted?) -
Immunoprecipitation - Anti-TBL1 antibody - ChIP Grade (ab24548)TBL1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TBL1 - ChIP Grade (ab24548) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab24548.
Secondary: Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 64kDa: TBL1. -
Immunocytochemistry/ Immunofluorescence - TBL1 antibody - ChIP Grade (ab24548)This image is courtesy of an anonymous Abreviewab24548 staining TBL1 (green) in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
プロトコール
参考文献
This product has been referenced in:
- Stoy C et al. Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy. EMBO Mol Med 7:1048-62 (2015). ChIP . Read more (PubMed: 26070712) »
- Chen Y et al. TBLR1 fuses to retinoid acid receptor a in a variant t(3;17)(q26;q21) translocation of acute promyelocytic leukemia. Blood 124:936-45 (2014). Read more (PubMed: 24782508) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"