The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml.
Use at an assay dependent concentration.
Antibody detection limit dilution 1:4,000.
Use a concentration of 3 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human TBL1 peptide (ab22881).
F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
Belongs to the WD repeat EBI family. Contains 1 F-box-like domain. Contains 1 LisH domain. Contains 8 WD repeats.
The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling TBL1 with ab5642 at 4 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
Western blot - Anti-TBL1 antibody (ab2243)
ab2243 staining (4µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane).Primary incubated for 1 hour.Detected by western blot using chemiluminescence. ab2243 staining (4µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.