製品の概要

  • 製品名Anti-TBC1D4 antibody
    TBC1D4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TBC1D4
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1250 to the C-terminus of Human TBC1D4.

    (Peptide available as ab26327.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human skeletal muscle tissue lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 147 kDa).
IP Use at an assay dependent concentration. PubMed: 20937822
IHC-P 1/135. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
  • 組織特異性Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
  • 配列類似性Contains 2 PID domains.
    Contains 1 Rab-GAP TBC domain.
  • 翻訳後修飾Phosphorylated by AKT1; insulin-induced.
    Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
    Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
  • 細胞内局在Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acrg embryonic lethality (mouse) minimal region ortholog antibody
    • Acrg embryonic lethality minimal region ortholog antibody
    • Acrg embryonic lethality mouse minimal region ortholog antibody
    • Akt substrate of 160 kDa antibody
    • AS 160 antibody
    • AS160 antibody
    • BUB2 antibody
    • CDC16 antibody
    • KIAA0603 antibody
    • TBC (Tre 2 BUB2 CDC16) domain containing protein antibody
    • TBC Tre 2 BUB2 CDC16 domain containing protein antibody
    • TBC1 D4 antibody
    • TBC1 domain family member 4 antibody
    • Tbc1d4 antibody
    • TBCD4_HUMAN antibody
    • Tre-2 antibody
    see all

Anti-TBC1D4 antibody 画像

  • All lanes : Anti-TBC1D4 antibody (ab24469) at 0.46 µg/ml

    Lane 1 : Human skeletal muscle cell lysate at 10 µg
    Lane 2 : Human skeletal muscle cell lysate at 20 µg
    Lane 3 : Human skeletal muscle cell lysate at 40 µg
    Lane 4 : Human skeletal muscle cell lysate at 60 µg

    Secondary
    Anti-rabbit HRP

    Predicted band size : 147 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of Jonas Treebak

    Incubation with the primary antibody was at 4 degrees celsius overnight. The secondary anti-rabbit HRP antibody was incubated for one hour at room temperature.
  • Image courtesy of Human Protein Atlas

    ab24469 staining TBC1D4 in human stomach. Paraffin embedded human stomach tissue was incubated with ab24469 (1/135 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab24469 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • Anti-TBC1D4 antibody (ab24469) at 1 µg/ml + TBC1D4 Human Partial Recombinant Protein (His Tagged) at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 147 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)

Anti-TBC1D4 antibody (ab24469) 使用論文

This product has been referenced in:
  • Kim TY  et al. Substrate trapping proteomics reveals targets of the ßTrCP2/FBXW11 ubiquitin ligase. Mol Cell Biol 35:167-81 (2015). WB ; Human . Read more (PubMed: 25332235) »
  • Treebak JT  et al. Acute exercise and physiological insulin induce distinct phosphorylation signatures on TBC1D1 and TBC1D4 proteins in human skeletal muscle. J Physiol 592:351-75 (2014). Human . Read more (PubMed: 24247980) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Skeletal Muscle)
Loading amount 50 µg
Specification Skeletal Muscle
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 May 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Sample Human Tissue lysate - whole (skeletal muscle)
Specification skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Mr. Jonas Treebak

Verified customer

投稿 Nov 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"