製品の概要

  • 製品名
    Anti-TBC1D21 antibody
  • 製品の詳細
    Rabbit polyclonal to TBC1D21
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (TTLSPENSLS ARQSASFILV KRKPPIDKTE WDSFFDESGH LAKSRDFICV) of human TBC1D21 (NP_699187).

  • ポジティブ・コントロール
    • Human fetal brain lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.Can be blocked with TBC1D21 peptide (ab184002). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

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  • Anti-TBC1D21 antibody (ab89799) at 1 µg/ml (5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <22 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

This product has been referenced in:
  • Lin YH  et al. RAB10 Interacts with the Male Germ Cell-Specific GTPase-Activating Protein during Mammalian Spermiogenesis. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28067790) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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