Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.Can be blocked with TBC1D21 peptide (ab184002). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Anti-TBC1D21 antibody 画像
Western blot - TBC1D21 antibody (ab89799)
Anti-TBC1D21 antibody (ab89799) at 1 µg/ml (5% skim milk / PBS buffer) + human fetal brain lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
39 kDaObserved band size :
42 kDa (why is the actual band size different from the predicted?
)Additional bands at :
<22 kDa. We are unsure as to the identity of these extra bands.
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