製品の概要

  • 製品名Anti-TAZ antibody
    TAZ 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TAZ
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, surrounding amino acids 386-390 (VESAL) of Human TAZ.

  • ポジティブ・コントロール
    • Jurkat and HeLa cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.

ターゲット情報

  • 機能Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
  • 組織特異性Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
  • 配列類似性Contains 1 WW domain.
  • ドメインThe PDZ-binding motif is essential for stimulated gene transcription. It localizes the protein into both punctate nuclear foci and plasma membrane-associated complexes.
    Binds to transcription factors via its WW domain.
  • 翻訳後修飾Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export.
  • 細胞内局在Nucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZP586I1419 antibody
    • FLJ27004 antibody
    • FLJ45718 antibody
    • OTTHUMP00000215994 antibody
    • OTTHUMP00000215995 antibody
    • OTTHUMP00000215996 antibody
    • OTTHUMP00000216001 antibody
    • TAZ antibody
    • Transcriptional co activator with PDZ binding motif antibody
    • Transcriptional coactivator with PDZ binding motif antibody
    • Transcriptional coactivator with PDZ-binding motif antibody
    • WW domain containing transcription regulator 1 antibody
    • WW domain containing transcription regulator protein 1 antibody
    • WW domain-containing transcription regulator protein 1 antibody
    • WWTR 1 antibody
    • WWTR1 antibody
    • WWTR1_HUMAN antibody
    see all

Anti-TAZ antibody 画像

  • All lanes : Anti-TAZ antibody (ab119373) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 44 kDa

Anti-TAZ antibody (ab119373) 使用論文

ab119373 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (N1E-115 murine neuroblastoma)
Specification N1E-115 murine neuroblastoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 4%
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Verified customer

投稿 Feb 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Loading amount 50000 cells
Specification MDA-MB-231 breast cancer cell line
Gel Running Conditions Reduced Denaturing (10% PAGE)
Blocking step 2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Thank you for your response.



This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial ofab119373 ((exchange for the original ab84927)) as a free of charge replacement. I hope ...

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Thank you for your enquiry and your interest in our products.   I have conducted a search for you and for your customer and 4 antibodies come up under TAZ target name using the advanced search engine for primary antibodies:   -  ab118373, -...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"