Anti-TATA binding protein TBP 抗体 [58C9] (ab61411)

製品の概要

  • 製品名Anti-TATA binding protein TBP antibody [58C9]
    TATA binding protein TBP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [58C9] to TATA binding protein TBP
  • 特異性ab61411 recognises TATA binding protein TBP also in Sf9 cells.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human, Saccharomyces cerevisiae, Drosophila melanogaster, Corn
  • 免疫原

    Drosophila TFIID complex.

  • ポジティブ・コントロール
    • Nuclear extracts of D melanogaster cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
  • 組織特異性Widely expressed, with levels highest in the testis and ovary.
  • 関連疾患Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • 配列類似性Belongs to the TBP family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • GTF2D antibody
      • GTF2D1 antibody
      • HDL4 antibody
      • MGC117320 antibody
      • MGC126054 antibody
      • MGC126055 antibody
      • SCA17 antibody
      • TATA binding factor antibody
      • TATA box factor antibody
      • TATA sequence binding protein antibody
      • TATA sequence-binding protein antibody
      • TATA-binding factor antibody
      • TATA-box binding protein N-terminal domain antibody
      • TATA-box factor antibody
      • TATA-box-binding protein antibody
      • TBP antibody
      • TBP_HUMAN antibody
      • TFIID antibody
      • Transcription initiation factor TFIID TBP subunit antibody
      see all

    Anti-TATA binding protein TBP antibody [58C9] 画像

    • Lane 1 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 2 µg/ml
      Lane 2 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 1 µg/ml
      Lane 3 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 0.5 µg/ml
      Lane 4 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 0.2 µg/ml
      Lane 5 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) (Negative control )

      Lane 1 : Nuclear cell extract of D.Mel cells
      Lane 2 : Nuclear cell extract of D.Mel cells
      Lane 3 : Nuclear cell extract of D.Mel cells
      Lane 4 : Nuclear cell extract of D.Mel cells
      Lane 5 : Nuclear cell extract of D.Mel cells

      Lysates/proteins at 20 µg/ml per lane.

      Secondary
      Goat Anti-Mouse – Peroxidase conjugate
      Developed using the ECL technique

      Predicted band size : 38 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)

    Anti-TATA binding protein TBP antibody [58C9] (ab61411) 使用論文

    This product has been referenced in:
    • Li L  et al. A specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation. Elife 4:e08536 (2015). Read more (PubMed: 26393420) »
    • von Marschall Z & Fisher LW Secreted Frizzled-related protein-2 (sFRP2) augments canonical Wnt3a-induced signaling. Biochem Biophys Res Commun 400:299-304 (2010). WB ; Human . Read more (PubMed: 20723538) »

    See all 3 Publications for this product

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