Anti-Tartrate Resistant Acid Phosphatase 抗体 [EPR15556] (ab191406)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
- Suitable for: IP, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]
Tartrate Resistant Acid Phosphatase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase -
由来種
Rabbit -
特異性
This antibody gave negative results in IHC-P in both mouse and rat species. Both species were also tested in WB with positive results. Please do not hesitate to contact our Scientific Support if you have any questions. -
アプリケーション
適用あり: IP, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- A431, MDA-MB-435, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates; Human tonsil and fetal kidney lysates; Human tonsil tissue; MDA-MB-435 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15556 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab191406の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/50.
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ICC/IF |
1/100.
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WB |
1/1000 - 1/10000. Detects a band of approximately 35, 42 kDa (predicted molecular weight: 37 kDa).
Per the literature, a novel previously unreported 42 kDa variant of the Tartrate Resistant Acid Phosphatase 5a isoform was identified. So the 42 kDa band should be the Tartrate Resistant Acid Phosphatase 5a isoform. |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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IP
1/50. |
ICC/IF
1/100. |
WB
1/1000 - 1/10000. Detects a band of approximately 35, 42 kDa (predicted molecular weight: 37 kDa). Per the literature, a novel previously unreported 42 kDa variant of the Tartrate Resistant Acid Phosphatase 5a isoform was identified. So the 42 kDa band should be the Tartrate Resistant Acid Phosphatase 5a isoform. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. -
配列類似性
Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 54 Human
- Entrez Gene: 11433 Mouse
- Entrez Gene: 25732 Rat
- Omim: 171640 Human
- SwissProt: P13686 Human
- SwissProt: Q05117 Mouse
- SwissProt: P29288 Rat
- Unigene: 1211 Human
see all -
別名
- Type 5 acid phosphatase antibody
- Acid phosphatase 5 tartrate resistant antibody
- Acid phosphatase 5, tartrate resistant antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : MDA-MB-435 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 35,42 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM /TBST.
Per the literature, a novel previously unreported 42 kDa variant of the TRAP 5a isoform was identified. So the 42 kDa band should be the TRAP 5a isoform.
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All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM /TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized MDA-MB-435 cells labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).
The two negative controls used anti-TRAP 5 primary antibody at 1/100 dilution, followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
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Western blot analysis of TRAP 5 in MDA-MB-435 cell lysate immunoprecipitated using ab191406 at 1/50 dilution (Lane 1). Lane 2: PBS instead of MDA-MB-435 lysates.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (26)
ab191406 は 26 報の論文で使用されています。
- Kim M & Park M The Brown Algae Ishige sinicola Extract Ameliorates Ovariectomy-Induced Bone Loss in Rats and Suppresses Osteoclastogenesis through Downregulation of NFATc1/c-Fos. Nutrients 14:N/A (2022). PubMed: 35565651
- Cai P et al. Carnosol inhibits osteoclastogenesis in vivo and in vitro by blocking the RANKL‑induced NF‑κB signaling pathway. Mol Med Rep 26:N/A (2022). PubMed: 35593299
- Chen Q et al. Aberrant activation of TGF-β1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann disease. Front Endocrinol (Lausanne) 13:913979 (2022). PubMed: 36325441
- Zhang Y et al. Complete abrogation of key osteoclast markers with a membrane-anchored tissue inhibitor of metalloproteinase : a novel approach in the prevention of osteoclastogenesis. Bone Joint Res 11:763-776 (2022). PubMed: 36331083
- Jiang C et al. Oxymatrine Attenuates Osteoclastogenesis via Modulation of ROS-Mediated SREBP2 Signaling and Counteracts Ovariectomy-Induced Osteoporosis. Front Cell Dev Biol 9:684007 (2021). PubMed: 34136493