Anti-Tartrate Resistant Acid Phosphatase 抗体 [26E5] (ab49507)


  • 製品名Anti-Tartrate Resistant Acid Phosphatase antibody [26E5]
    Tartrate Resistant Acid Phosphatase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [26E5] to Tartrate Resistant Acid Phosphatase
  • 特異性This antibody is specific for Tartrate Resistant Acid Phosphatase.
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
    適用なし: IHC-Fr or WB
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fusion protein corresponding to the N terminal portion of human Tartrate Resistant Acid Phosphatase (TRAP)

  • ポジティブ・コントロール
    • Tonsil, Human osteoclastoma



Our Abpromise guarantee covers the use of ab49507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We recommend high temperature antigen unmasking and 60 minutes primary antibody incubation at 25°C prior to commencing with IHC staining protocol.

ICC/IF 1/1000.
  • 追加情報Is unsuitable for IHC-Fr or WB.
  • ターゲット情報

    • 機能Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
    • 配列類似性Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
    • 細胞内局在Lysosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Type 5 acid phosphatase antibody
      • Acid phosphatase 5 tartrate resistant antibody
      • Acid phosphatase 5, tartrate resistant antibody
      • ACP5 antibody
      • EC antibody
      • MGC117378 antibody
      • PPA5_HUMAN antibody
      • serum band 5 tartrate-resistant acid phosphatase antibody
      • SPENCDI antibody
      • T5ap antibody
      • Tartrate resistant acid ATPase antibody
      • Tartrate resistant acid phosphatase type 5 antibody
      • Tartrate resistant acid phosphatase type 5 precursor antibody
      • Tartrate-resistant acid ATPase antibody
      • Tartrate-resistant acid phosphatase type 5 antibody
      • TR AP antibody
      • TR-AP antibody
      • TRACP 5 antibody
      • TRAP antibody
      • TrATPase antibody
      • Type 5 acid phosphatase antibody
      see all

    Anti-Tartrate Resistant Acid Phosphatase antibody [26E5] 画像

    • ab49507 staining Tartate Resistance Acid Phosphatase in Human osteoclast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/100 in 0.1% Triton + 3% BSA) for 12 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemical staining (Paraffin section) for Tartrate Resistant Acid Phosphatase using ab49507 with Human osteoclastoma as positive control. Note intense cytoplasmic staining of osteoclasts.
    • ICC/IF image of ab49507 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Tartrate Resistant Acid Phosphatase antibody [26E5] (ab49507) 使用論文

    This product has been referenced in:
    • Kawamura M  et al. Clinical significance of tartrate-resistant acid phosphatase type-5 expression in human gastric cancer. Anticancer Res 34:3425-9 (2014). IHC-P ; Human . Read more (PubMed: 24982350) »
    • Xia L  et al. ACP5, a direct transcriptional target of FoxM1, promotes tumor metastasis and indicates poor prognosis in hepatocellular carcinoma. Oncogene N/A:N/A (2013). Read more (PubMed: 23604121) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Sample Human Cell (osteoclast)
    Specification osteoclast
    Permeabilization Yes - 0.1% TritonX-100
    Fixative Paraformaldehyde

    Rekha Rao Manepalli

    Verified customer

    投稿 Sep 24 2013

    Oui, n'hésitez pas à me contacter de nouveau si vous avez plus d'informations concernant la différence entre la forme 5a et 5b, je serai ravi de chercher l'anticorps qui convient à vos travaux.

    Merci de nous avoir contactés.

    Les quatre anticorps anti-TRAP (type 5)que nous avons sont :
    Ils sont tous dirigés vers la protéine dont la référence UniProt...

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    Thank you for your reply. As I mentioned previously, we do not give free samples of our antibodies, even for testing purposes. We issue testing discount codes for antibodies that have not been tested in a specific application or species. How the testin...

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    Thank you for your reply. As mentioned in the previous emails, we do not send samples sizes of our antibodies, under our Abpomise we guarantees the antibodies to work as stated on the datasheet. If the antibodies do not work as stated then we will eith...

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    Thank you for your reply. I am sorry to confirm that as far as we are aware, ab49507 has not been tested with samples from mouse. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as s...

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    Thank you for your enquiry. I am pleased to confirm that ab49507 Tartrate Resistant Acid Phosphatase antibody has already been tested and is guaranteed in IHC-P. Therefore, I am sorry that a testing discount to test in IHC-P would not be appropriat...

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