製品の概要

  • 製品名Anti-TAPA1 antibody [1D6]
    TAPA1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1D6] to TAPA1
  • 特異性ab35026 recognises CD81, a 26kD cell surface antigen and a member of the tetraspanin family.
  • アプリケーション適用あり: Flow Cyt, IHC-P, IP, WB, Functional Studies, ICC/IFmore details
  • 種交差性
    交差種: Human, Chimpanzee
  • 免疫原

    Tissue, cells or virus corresponding to Human TAPA1. Aggregated OCI-LY8 cells.

  • 特記事項


    Ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects. Endotoxin Level: <0.01 Eu/ug (low endotoxin grade).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody was purified from tissue culture supernatant.
  • 一次抗体 備考Ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects. Endotoxin Level: <0.01 Eu/ug (low endotoxin grade).
  • ポリ/モノモノクローナル
  • クローン名1D6
  • ミエローマP3x63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab35026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 26 kDa.

PMID:20224111

Functional Studies Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
  • 組織特異性Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
  • 関連疾患Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 配列類似性Belongs to the tetraspanin (TM4SF) family.
  • 翻訳後修飾Not glycosylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 26 kDa cell surface protein TAPA 1 antibody
    • 26 kDa cell surface protein TAPA-1 antibody
    • 26 kDa cell surface protein TAPA1 antibody
    • CD 81 antibody
    • CD81 antibody
    • CD81 antigen (target of antiproliferative antibody 1) antibody
    • CD81 antigen antibody
    • CD81 molecule antibody
    • CD81_HUMAN antibody
    • CVID6 antibody
    • S5.7 antibody
    • TAPA 1 antibody
    • TAPA1 antibody
    • Target of the antiproliferative antibody 1 antibody
    • Tetraspanin 28 antibody
    • Tetraspanin-28 antibody
    • Tetraspanin28 antibody
    • Tspan 28 antibody
    • Tspan-28 antibody
    • Tspan28 antibody
    see all

Anti-TAPA1 antibody [1D6] 画像

  • ab35026 staining TAPA1 in SH-SY5Y were transfected with cDNA encoding CD81 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 3% BSA for 2 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 3% BSA) for 2 hours. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Flow cytometry analysis of Human peripheral blood lymphocytes, staining TAPA1 with ab35026 at 1/10 dilution.

Anti-TAPA1 antibody [1D6] (ab35026) 使用論文

This product has been referenced in:
  • Drummond HA  et al. Renal inflammation and elevated blood pressure in a mouse model of reduced {beta}-ENaC. Am J Physiol Renal Physiol 301:F443-9 (2011). WB ; Mouse . Read more (PubMed: 21543417) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SH-SY5Y were transfected with cDNA encoding CD81)
Permeabilization Yes - 0,2% Triton PBS
Specification SH-SY5Y were transfected with cDNA encoding CD81
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Jul 29 2015

Thank you for contacting us today in regards to the Anti-TAPA1 antibody [1D6] - Azide free (ab35026).

As discussed over the phone, I have checked into whether the antibody has been used in ICC. I can confirm that the antibody has been used in ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"