製品の概要

  • 製品名Anti-TAP1 antibody
    TAP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to TAP1
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Recombinant fragment with tag: GSETRRLSLF LVLVVLSSLG EMAIPFFTGR LTDWILQDGS ADTFTRNLTL MSILTIASAV LEFVGDGIYN NTMGHVHSHL QGEVFGAVLR QETEFFQQNQ TGNIMSRVTE , corresponding to amino acids 241-350 of Human TAP1

  • ポジティブ・コントロール
    • TAP1 transfected 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG3
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: Use at an assay dependent dilution. Sandwich ELISA - The detection limit for recombinant tagged TAP1 is approximately 3ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
    • 関連疾患Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
    • 配列類似性Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
      Contains 1 ABC transmembrane type-1 domain.
      Contains 1 ABC transporter domain.
    • ドメインThe peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
    • 細胞内局在Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ABC 17 antibody
      • ABC transporter MHC 1 antibody
      • ABC17 antibody
      • ABCB 2 antibody
      • ABCB2 antibody
      • Antigen peptide transporter 1 antibody
      • APT 1 antibody
      • APT1 antibody
      • ATP binding cassette sub family B (MDR/TAP) member 2 antibody
      • ATP binding cassette sub family B member 2 antibody
      • ATP binding cassette transporter antibody
      • ATP-binding cassette sub-family B member 2 antibody
      • D6S114E antibody
      • FLJ26666 antibody
      • FLJ41500 antibody
      • Peptide supply factor 1 antibody
      • Peptide transporter involved in antigen processing 1 antibody
      • Peptide transporter PSF 1 antibody
      • Peptide transporter PSF1 antibody
      • Peptide transporter TAP 1 antibody
      • Peptide transporter TAP1 antibody
      • PSF 1 antibody
      • PSF-1 antibody
      • PSF1 antibody
      • Really interesting new gene 4 protein antibody
      • RING 4 antibody
      • RING4 antibody
      • TAP 1 antibody
      • TAP1 antibody
      • TAP1*0102N antibody
      • TAP1_HUMAN antibody
      • TAP1N antibody
      • Transporter 1 ATP binding cassette sub family B (MDR/TAP) antibody
      • Transporter 1 ATP binding cassette sub family B antibody
      • Transporter associated with antigen processing antibody
      • Transporter ATP binding cassette major histocompatibility complex 1 antibody
      • Y3 antibody
      see all

    Anti-TAP1 antibody 画像

    • All lanes : Anti-TAP1 antibody (ab66074) at 1 µg/ml

      Lane 1 : TAP1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate.

      Lysates/proteins at 50 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 81 kDa
      Observed band size : 81 kDa
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Anti-TAP1 antibody (ab66074) 使用論文

    ab66074 has not yet been referenced specifically in any publications.

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