製品の概要
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製品名Anti-TAP1 antibody
TAP1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TAP1
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特異性This antibody detects an ~70 kDa protein, corresponding to the apparent molecular mass of Transporter Associated with Antigen Processing 1 (TAP1) on SDS-PAGE immunoblots. The immunogen sequence used to generate this antibody is based on the sequence of ATP-binding cassette sub-family B member 2.
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アプリケーション適用あり: WB, IP, ICCmore details
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種交差性交差種: Rat, Human
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免疫原
Synthetic peptide:
CYWAMVQAPADAPE
, corresponding to amino acids 735-748 of Human TAP1 (member 2). -
ポジティブ･コントロール
- HeLa cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: Whole Serum -
精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab13516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/4000. Detects a band of approximately 70 kDa. | |
| IP | 1/100. | |
| ICC | Use at an assay dependent dilution. |
ターゲット情報
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機能Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
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関連疾患Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
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配列類似性Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain. -
ドメインThe peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
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細胞内局在Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 6890 Human
- Entrez Gene: 24811 Rat
- Omim: 170260 Human
- SwissProt: Q03518 Human
- SwissProt: P36370 Rat
- Unigene: 352018 Human
- Unigene: 10763 Rat
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別名
- ABC 17 antibody
- ABC transporter MHC 1 antibody
- ABC17 antibody
see all
プロトコール
Anti-TAP1 antibody (ab13516) 使用論文
This product has been referenced in:
- Zhang SY et al. HMME-based PDT restores expression and function of transporter associated with antigen processing 1 (TAP1) and surface presentation of MHC class I antigen in human glioma. J Neurooncol 105:199-210 (2011). WB . Read more (PubMed: 21520005) »
- Larrea E et al. Oncostatin M enhances the antiviral effects of type I interferon and activates immunostimulatory functions in liver epithelial cells. J Virol 83:3298-311 (2009). WB ; Human . Read more (PubMed: 19158240) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"