製品の概要

  • 製品名Anti-TAOK3 antibody
    TAOK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TAOK3
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to Human TAOK3 (C terminal).
    Database link: Q9H2K8

  • ポジティブ・コントロール
    • HeLa whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 1-5µg/mg of lysate.
    WB: 1/5000 - 1/25000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
    • 組織特異性Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
    • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CTCL tumor antigen HD CL 09 antibody
      • CTCL-associated antigen HD-CL-09 antibody
      • Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
      • Cutaneous T-cell lymphoma-associated antigen HD-CL-09 antibody
      • Dendritic cell derived protein kinase antibody
      • Dendritic cell-derived protein kinase antibody
      • DKFZp666H245 antibody
      • DPK antibody
      • FLJ31808 antibody
      • hKFC A antibody
      • hKFC-A antibody
      • JIK antibody
      • JNK/SAPK inhibitory kinase antibody
      • JNK/SAPK-inhibitory kinase antibody
      • Jun kinase inhibitory kinase antibody
      • Jun kinase-inhibitory kinase antibody
      • KDS antibody
      • Kinase from chicken homolog A antibody
      • MAP3K18 antibody
      • Serine kinase antibody
      • Serine/threonine-protein kinase TAO3 antibody
      • STE20 like kinase antibody
      • TAO kinase 3 antibody
      • TAOK3 antibody
      • TAOK3_HUMAN antibody
      • Thousand and one amino acid protein 3 antibody
      see all

    Anti-TAOK3 antibody 画像

    • All lanes : Anti-TAOK3 antibody (ab70297) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg

      Developed using the ECL technique

      Predicted band size : 105 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Western Blot showing Lane 2: ab70297 staining JIK in HeLa whole cell lysates immunoprecipitated using ab70297 at 3µg/mg lysate (1mg/lane). Lane 1: control IgG used for immunoprecipitation.Detection: Chemiluminescence with an exposure time of 3 minutes

    Anti-TAOK3 antibody (ab70297) 使用論文

    ab70297 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Murine embryonic fibroblast)
    Gel Running Conditions Non-reduced Denaturing (gel 10%)
    Loading amount 20 µg
    Treatment siRNA trasnfection directed against TAOK3
    Specification Murine embryonic fibroblast
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Mr. Bastiaan Maes

    Verified customer

    投稿 May 02 2016

    Thank you for taking the time to complete our questionnaire as requested.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

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    Thank you for taking the time to contact us and for your telephone call yesterday afternoon. I appreciate your sending a message, I did in factunfortunately have your email address incorrectand am pleased to have confirmation of this.

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    Application Western blot
    Sample Mouse Tissue lysate - whole (Spleen)
    Loading amount 100 µg
    Specification Spleen
    Gel Running Conditions Reduced Denaturing (6% running section)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 May 18 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"