製品の概要

  • 製品名
    Anti-TAOK2 (phospho S181) antibody
    TAOK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TAOK2 (phospho S181)
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to TAOK2. Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser181 of TAO2.
    Database link: Q9UL54

  • ポジティブ・コントロール
    • Rat hippocampal lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 138 kDa).

ターゲット情報

  • 機能
    Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1, but not isoform 2, activates the JNK MAP kinase pathway through the specific activation of the upstream MKK3 and MKK6 kinases. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of IKKA, and thus NF-kappa-B activation, but not that of JNK. Phosphorylates itself, MBP, activated MAPK8 and tubulins. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization (By similarity). Both isoforms are involved in MAPK14 phosphorylation.
  • 組織特異性
    Ubiquitously expressed, with a higher level of expression in testis and brain.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Isoforms 1 and 2 are autophosphorylated.
    C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity.
    isoform 2 is phosphorylated at 'Ser-1031' by MAPK14. This phosphorylation is required PCDH8 for endocytosis.
  • 細胞内局在
    Cytoplasmic vesicle membrane. Cytoplasm > cytoskeleton. Nucleus. Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus and Cell projection > dendrite. In dendrites, colocalizes with PCDH8.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110033K02Rik antibody
    • B230344N16 antibody
    • hKFC C antibody
    • hKFC-C antibody
    • KIAA0881 antibody
    • Kinase from chicken homolog C antibody
    • MAP3K17 antibody
    • mKIAA0881 antibody
    • Prostate derived STE20 like kinase 1 antibody
    • Prostate derived STE20 like kinase PSK antibody
    • Prostate derived sterile 20 like kinase 1 antibody
    • Prostate-derived STE20-like kinase 1 antibody
    • PSK 1 antibody
    • PSK antibody
    • PSK-1 antibody
    • PSK1 antibody
    • PSK1 beta antibody
    • Serine/threonine protein kinase TAO2 antibody
    • Serine/threonine-protein kinase TAO2 antibody
    • TAO 1 antibody
    • TAO 2 antibody
    • TAO kinase 2 antibody
    • TAO1 antibody
    • TAO2 antibody
    • TAOK 2 antibody
    • Taok2 antibody
    • TAOK2_HUMAN antibody
    • Thousand and one amino acid protein 2 antibody
    • Thousand and one amino acid protein kinase antibody
    • UNQ2971/PRO7431 antibody
    see all

Anti-TAOK2 (phospho S181) antibody 画像

プロトコール

Anti-TAOK2 (phospho S181) antibody (ab32591) 使用論文

This product has been referenced in:
  • McNeer JL  et al. Arsenic trioxide-dependent activation of thousand-and-one amino acid kinase 2 and transforming growth factor-beta-activated kinase 1. Mol Pharmacol 77:828-35 (2010). WB ; Human . Read more (PubMed: 20159944) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryos)
Specification
0-4hr embryos
Treatment
control shRNA and Tao shRNA embryos
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Richelle Sopko

Verified customer

投稿 Mar 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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