Anti-Talin 1 抗体 (ab71333)
Key features and details
- Rabbit polyclonal to Talin 1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Talin 1 antibody
Talin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Talin 1 -
由来種
Rabbit -
特異性
Replenishment batches of our polyclonal antibody, ab71333 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab108480.
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アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken -
免疫原
Synthetic peptide corresponding to Human Talin 1 aa 1650-1750 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab71332) -
ポジティブ・コントロール
- ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71333の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P | (1) |
Use a concentration of 5 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 270 kDa (predicted molecular weight: 270 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 270 kDa (predicted molecular weight: 270 kDa). |
ターゲット情報
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機能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. -
配列類似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
細胞内局在
Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 395194 Chicken
- Entrez Gene: 7094 Human
- Entrez Gene: 21894 Mouse
- Entrez Gene: 313494 Rat
- Omim: 186745 Human
- SwissProt: P54939 Chicken
- SwissProt: Q9Y490 Human
- SwissProt: P26039 Mouse
see all -
別名
- ILWEQ antibody
- Talin 1 antibody
- Talin antibody
see all
画像
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ab71333 staining Talin 1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab71333 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Talin 1 antibody (ab71333) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 270 kDa
Observed band size: 270 kDa
Additional bands at: 100 kDa, 230 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
IHC image of Talin 1 staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71333, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse endoderm cell
Preparation:
Fix in 3% PFA in PBS for 30 min at RT
Primary antibody: Rabbit anti-talin (ab71333), 1:100
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1:100
Nuclear counterstain: DAPI
Rhodamine-phalloidin, 1:100
Nuclei were counterstained with DAPI
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All lanes : Anti-Talin 1 antibody (ab71333) at 1/1000 dilution
Lane 1 : Human platelets
Lane 2 : Mouse platelets
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to rabbit IgG conjugated to IRDye 800CW (undiluted)
Performed under reducing conditions.
Predicted band size: 270 kDa
Exposure time: 1 minute
Detection method: Licor System
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab71333 は 29 報の論文で使用されています。
- Hadjisavva R et al. Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition. Cell Rep 40:111091 (2022). PubMed: 35858563
- Huang J et al. Micro RNA miR-1303 Promotion of Proliferation, Migration and Invasion of Human Liver Cancer Cells Is Enhanced by Low Talin 1 (TLN1) Expression. Anticancer Res 42:4715-4725 (2022). PubMed: 36192016
- Zhou DW et al. Force-FAK signaling coupling at individual focal adhesions coordinates mechanosensing and microtissue repair. Nat Commun 12:2359 (2021). PubMed: 33883558
- Wang L et al. Combination CTLA-4 immunoglobulin treatment and ultrasound microbubble-mediated exposure improve renal function in a rat model of diabetic nephropathy. Aging (Albany NY) 13:8524-8540 (2021). PubMed: 33714204
- Alday-Parejo B et al. MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis. Cell Adh Migr 15:126-139 (2021). PubMed: 33823745