製品の概要

  • 製品名Anti-Talin 1 antibody
    Talin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Talin 1
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment: SALSVVQNLE KDLQEVKAAA RDGKLKPLPG ETMEKCTQDL GNSTKAVSSA IAQLLGEVAQ GNENYAGIAA RDVAGGLRSL AQAARGVAAL TSDPAVQA, corresponding to amino acids 1052-1150 of Human Talin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG3
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 270 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells. ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
  • 配列類似性Contains 1 FERM domain.
    Contains 1 I/LWEQ domain.
  • 細胞内局在Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).
  • Information by UniProt
  • 参照データベース
  • 別名
    • ILWEQ antibody
    • Talin 1 antibody
    • Talin antibody
    • Talin-1 antibody
    • TLN 1 antibody
    • TLN antibody
    • Tln1 antibody
    • TLN1_HUMAN antibody
    see all

Anti-Talin 1 antibody 画像

  • Talin antibody (ab57758) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.


  • Predicted band size : 270 kDa
  • ICC/IF image of ab57758 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57758, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing RAW 264.7 cells stained with ab57758 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57758, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in RAW 264.7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Talin 1 antibody (ab57758) 使用論文

This product has been referenced in:
  • Dai S  et al. Fine tuning inflammation at the front door: macrophage complement receptor 3-mediates phagocytosis and immune suppression for Francisella tularensis. PLoS Pathog 9:e1003114 (2013). ICC/IF ; Human . Read more (PubMed: 23359218) »
  • Kahner BN  et al. Kindlins, Integrin Activation and the Regulation of Talin Recruitment to aIIbß3. PLoS One 7:e34056 (2012). WB . Read more (PubMed: 22457811) »

See all 3 Publications for this product

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for the additional information! I would also suggest to use more primary antibody as the suggested dilution range is 1 - 5 ug/ml (1/500 to 1/100). This could help as well with obtaining a signal. As far as detecting actin, you would ne...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monito...

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The antibody has not, to our knowledge, been tested on Jurkat cells. However, reactivity with human samples in general is covered by our Abpromise guarantee: if the antibody fails to detect talin in human samples using one of the tested applications li...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"