製品の概要

  • 製品名Anti-TAK1 antibody
    TAK1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to TAK1
  • 特異性This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1).
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Xenopus laevis, Orangutan
  • 免疫原

    Synthetic peptide:

    C-AELDQDEKDQQNT

    , corresponding to internal sequence amino acids 552-564 of Human TAK1

  • ポジティブ・コントロール
    • Lysates from cell lines: NIH/3T3 and A431.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab50431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 67 kDa). Approx 75kDa band observed in lysates of cell lines NIH/3T3 and A431.
ELISA Use at an assay dependent dilution. Antibody detection limit dilution 1:1,000.

ターゲット情報

  • 機能Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
    Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
  • Information by UniProt
  • 参照データベース
  • 別名
    • M3K7_HUMAN antibody
    • MAP3K 7 antibody
    • Map3k7 antibody
    • MEKK7 antibody
    • Mitogen activated protein kinase kinase kinase 7 antibody
    • Mitogen-activated protein kinase kinase kinase 7 antibody
    • TAK1 antibody
    • TGF beta activated kinase 1 antibody
    • TGF-beta-activated kinase 1 antibody
    • TGF1a antibody
    • Transforming growth factor beta activated kinase 1 antibody
    • Transforming growth factor-beta-activated kinase 1 antibody
    see all

Anti-TAK1 antibody 画像

  • Anti-TAK1 antibody (ab50431) at 1 µg/ml + HeLa cell lysate (35µg protein in RIPA buffer).

    Predicted band size : 67 kDa
    Observed band size : 76 kDa (why is the actual band size different from the predicted?)
  • Anti-TAK1 antibody (ab50431) at 0.1 µg/ml + Cell lysates prepared from NIH/3T3 cells at 35 µg
    Developed using the ECL technique

    Predicted band size : 67 kDa

Anti-TAK1 antibody (ab50431) 使用論文

ab50431 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (ccl13)
Loading amount 30 µg
Specification ccl13
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Primary T cells)
Loading amount 30 µg
Specification Primary T cells
Treatment LPS
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Karppiah Muthumani

Verified customer

投稿 Apr 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"