The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.
Belongs to the TAF8 family. Contains 1 histone-fold domain.
Ectopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition.
Nucleus. Cytoplasm. Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell.
Detection of Human TAF8 by Western blot of Immunoprecipitate.
TAF8 was immunoprecipitated from 293T whole cell lysate, using ab114114 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). ab114114 was used for the subsequent WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.