The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
Use a concentration of 1 µg/ml.
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
Belongs to the TAF7 family.
The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which methylates Lys-5 in vitro.
Overlay histogram showing HeLa cells stained with ab57494 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57494, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
TAF7 antibody (ab57494) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymph node.
Western blot - Anti-TAF7 antibody (ab57494)
Predicted band size : 40 kDa TAF7 antibody (ab57494) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
Western blot - TAF7 antibody (ab57494)Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
Lane 2 : Anti-TAF7 antibody (ab57494) at 1/1250 dilution Lane 3 : Anti-TAF7 antibody (ab57494) at 1/2500 dilution Lane 4 : Anti-TAF7 antibody (ab57494) at 1/5000 dilution Lane 5 : Anti-TAF7 antibody (ab57494) at 1/10000 dilution
Lane 2 : whole cell lysate prepared from SW780 badder cancer cells Lane 3 : whole cell lysate prepared from SW780 badder cancer cells Lane 4 : whole cell lysate prepared from SW780 badder cancer cells Lane 5 : whole cell lysate prepared from SW780 badder cancer cells
Lysates/proteins at 25 µg per lane.
Secondary Goat anti-mouse IgG conjugated to HRP Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 40 kDa Observed band size : 40 kDa Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
Lane 1: All Blue Biorad.Gel run under denaturing conditions with a 4-12% gradient.Blocking step performed using 5% milk for one hour at room temperature.Primary antibody incubated for 16 hours.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TAF7 antibody (ab57494)This image was kindly supplied by Dr Karin Birkenkamp-Demtroeder by Abreview
ab57494 staining TAF7 in human bladder cancer tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TEG. Samples were then blocked with 1% serum for 1 hour at room temperature followed by incubation with the primary antibody at a 1/3500 dilution for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.
Gegonne A et al. The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. Mol Cell Biol32:1984-97 (2012).
Read more (PubMed: 22411629) »
Devaiah BN & Singer DS Cross-talk Among RNA Polymerase II Kinases Modulates C-terminal Domain Phosphorylation. J Biol Chem287:38755-66 (2012).
Read more (PubMed: 23027873) »