製品の概要

  • 製品名Anti-TAF6 antibody
    TAF6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TAF6
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residue 627 and 677 of human TAF6 (NP_005632.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性TAF6 is a subunit of transcription factor IID (TFIID). Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • TAF2E antibody
    • E 70/85kD antibody
    • TAF 6 antibody
    • TAF6 RNA polymerase II TATA box binding protein (TBP) associated factor 80 kD antibody
    • TAF6 RNA polymerase II, TATA box binding protein associated factor, 80kDa antibody
    • TAFII 70 antibody
    • TAFII80 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II E 70/85kD antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase II, antibody
    • TBP associated factor 6 antibody
    • Transcription initiation factor TFIID 70 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 6 antibody
    see all

Anti-TAF6 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TAF6 with ab76922 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TAF6 antibody (ab76922) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 73 kDa
    Observed band size : 94 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Detection of TAF6 by Western Blot of Immunprecipitate.
    ab76922 at 1µg/ml staining TAF6 in HeLa whole cell lysate immunoprecipitated using ab76922 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-TAF6 antibody (ab76922) 使用論文

ab76922 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"