Anti-Taf4 抗体 (ab63910)
Key features and details
- Rabbit polyclonal to Taf4
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
製品の概要
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製品名
Anti-Taf4 antibody -
製品の詳細
Rabbit polyclonal to Taf4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Recombinant fragment which was over-expressed in E.coli using a plasmid carrying the N-terminal domain of Taf4 protein (1-200aa) of Saccharomyces cerevisiae and purified by chromatography.
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ポジティブ・コントロール
- Extract of budding yeast cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63910の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa). |
ターゲット情報
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機能
Part of the TFIID complex, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone. -
配列類似性
Belongs to the TAF4 family.
Contains 1 TAFH (NHR1) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- SwissProt: P50105 Saccharomyces cerevisiae
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別名
- RNA polymerase II TBP-associated factor subunit C antibody
- TAF(II)130 antibody
- TAF(II)135 antibody
see all
画像
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All lanes : Anti-Taf4 antibody (ab63910) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Extract of budding yeast
Predicted band size: 42 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab63910 は 1 報の論文で使用されています。
- Seizl M et al. Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization. Nucleic Acids Res : (2011). WB ; Saccharomyces cerevisiae . PubMed: 21498544